核农学报2025,Vol.39Issue(8):1797-1806,10.DOI:10.11869/j.issn.1000-8551.2025.08.1797
盐胁迫下外源脱落酸对鹰嘴紫云英种子萌发及幼苗生理特性的影响
Effects of Exogenous Abscisic Acid on Seed Germination and Seedling Physiological Characteristics of Astragalus cicer Seedlings under Salt Stress
摘要
Abstract
To investigate the effects of ABA on seed germination and seedling physiological characteristics of Astragalus cicer seedlings under salt stress.Astragalus cicer cv.Ganlv No.2 was used as experimental material.Under salt stress(200 mmol·L-1 NaCl),6 concentrations of exogenous ABA(0,0.5,1,1.5,2 and 2.5 μmol·L-1)were applied during seed germination stage and 6 concentrations of exogenous ABA(0,5,10,20,40 and 60 μmol·L-1)were applied during the seedlings stage with two different application methods(leaf and root application).The seed germination and seedling physiological indexes were measured.The results showed that the appropriate concentration of exogenous ABA could improve the germination indexes and growth indexes of the seeds under salt stress,the 2.0 μmol·L-1 exogenous ABA had the best effect on the inhibition of A.cicer seed germination under salt stress.Compared with single salt stress,exogenous ABA treatment of 40 μmol·L-1 in leaves and 20 μmol·L-1 in roots significantly increased the contents of free proline,soluble sugar and soluble protein,inhibited the increase rate of relative conductivity and malondialdehyde content,and alleviated the damage of salt stress to cell membrane structure of seedlings.By calculating the membership function values of each index and analyzing the results,it was found that the 40 μmol·L-1 ABA had the best effect on alleviating the salt stress of A.cicer seedlings.In summary,the appropriate concentration of exogenous ABA can alleviate the damage of A.cicer seed germination and seedlings,and promote the growth of A.cicer seedlings under salt stress.The research results provide new ideas for the cultivation of A.cicer in saline alkali soil.关键词
鹰嘴紫云英/外源ABA/盐胁迫/种子萌发/生理特性Key words
Astragalus cicer/exogenous ABA/salt stress/seed germination/physiological characteristics引用本文复制引用
马福钦,王彦,郑晓琳,晋明慧,乃国栋,张玉娟,董文科..盐胁迫下外源脱落酸对鹰嘴紫云英种子萌发及幼苗生理特性的影响[J].核农学报,2025,39(8):1797-1806,10.基金项目
甘肃省科技厅自然科学基金项目资助(22JR5RA871) (22JR5RA871)