核农学报2025，Vol.39Issue(8)：1829-1839,11.DOI:10.11869/j.issn.1000-8551.2025.08.1829
苦豆子内生真菌对酿酒葡萄灰霉病菌的抑制作用
Antagonistic Activity of Endophytic Fungi from Sophora alopecuroides against Botrytis cinerea of Wine Grape
摘要
Abstract
Sophora alopecuroides is an important sandy medicinal plant in Northwest China,with rich endophytic fungal resources and biological activity.In order to explore the application of biological control technology in controlling the Botrytis cinerea of wine grapes,B.cinerea was used as the target to isolate and screen 4 strains of endophytic fungi with antagonistic effects from S.alopecuroides.Through the plate standoff method,the mycelial growth rate method,the two-sealed-base-plates method,optical microscopy observation,and the vitro grape berry experiment to explore the antibacterial mechanism and control effect of 4 strains of endophytic fungi from S.alopecuroide against B.cinerea.The results showed that all 4 strains of endophytic fungi from S.alopecuroide had significant inhibitory effects on B.cinerea.It was determined that the inhibitory rate of strain HG21(Fusarium solani)on the hyphae of B.cinerea was as high as 85.19％.When the concentration of the fermentation filtrate of strain HD31(Fusarium oxysporum)was 25％,and the inhibition rate of mycelial growth of B.cinerea was the highest at 42.46％.The volatile substances were produced after 6 days of pre-inoculation with strain BJ35(Alternaria alternata),and exhibited a significant inhibitory effect on the growth of B.cinerea with an inhibition rate of up to 88.01％.As affected by the inhibition of 4 strains of endophytic fungi from S.alopecuroides,the mycelial morphology of B.cinerea showed significant changes,such as swelling,breakage,desiccation,and twisting.Lastly,the vitro grape berry results demonstrated that the preventive and protective effects of 4 strains of endophytic fungi from S.alopecuroides in vitro grape berry were generally higher than their therapeutic and bactericidal effects.Moreover,the biocontrol effect of strain BD41(Alternaria alternata)was significantly higher than that of Trichoderma harzianum and difenoconazole.The findings suggest that the 4 strains of endophytic fungi from S.alopecuroides can effectively inhibit B.cinerea,which has provided biocontrol materials and theoretical references for the B.cinerea of wine grapes.关键词
苦豆子内生真菌/葡萄灰霉病菌/抑菌活性/生防效果Key words
Sophora alopecuroide endophytic fungi/Botrytis cinerea/antagonism/biological control引用本文复制引用
王一冬,文云,鞠明岫,王若彤,顾沛雯..苦豆子内生真菌对酿酒葡萄灰霉病菌的抑制作用[J].核农学报,2025,39(8):1829-1839,11.基金项目
宁夏回族自治区重点研发计划项目(2023BCF01026) （2023BCF01026）
