湖南农业大学学报（自然科学版）2025，Vol.51Issue(3)：16-23,8.DOI:10.13331/j.cnki.jhau.2025.03.003
马铃薯StZIP8-like基因的克隆及镉转运功能分析
Cloning of StZIP8-like gene in potato and analysis of its function in cadmium transport
摘要
Abstract
In order to explore the expression pattern and function of the StZIP8-like gene,the full-length sequence of the StZIP8-like gene was cloned from potato.The structure,physicochemical properties,and genetic relationship of this gene were predicted by bioinformatics methods.The expression pattern of this gene under cadmium(Cd)stress was analyzed using the RT-qPCR technique.A fusion expression vector was constructed to analyze the subcellular localization of the StZIP8-like protein in tobacco leaves.Through a yeast heterologous expression experiment,the Cd transport function of StZIP8-like was analyzed.The results of structural analysis showed that the full-length sequence of StZIP8-like was 1 044 bp,encoding 347 amino acids,containing a ZIP conserved domain and 7 transmembrane domains.Phylogenetic analysis revealed that the StZIP8-like protein had a close genetic relationship with the ZIP8 proteins in tomato and tobacco.The results of subcellular localization indicated that the StZIP8-like protein was localized in the plasma membrane.The RT-qPCR results showed that StZIP8-like was highly expressed in the roots of potato seedlings,and Cd stress induced the upregulation of the expression of the StZIP8-like gene in the roots,stems,and leaves of potato.The yeast experiment showed that the yeast with overexpression of StZIP8-like was more sensitive to Cd and had Cd absorption and transport activity.The above results indicate that StZIP8-like is induced by Cd stress and may be involved in the process of Cd absorption in potato.关键词
马铃薯/StZIP8-like/镉/基因表达/酵母Key words
potato/StZIP8-like/Cd/gene expression/yeast分类
农业科技引用本文复制引用
杨丽红,席银凯,王丹,胡新喜..马铃薯StZIP8-like基因的克隆及镉转运功能分析[J].湖南农业大学学报（自然科学版）,2025,51(3):16-23,8.基金项目
国家马铃薯产业技术体系(CARS-09-ES16) （CARS-09-ES16）
