郑州大学学报（医学版）2025，Vol.60Issue(4)：480-484,5.DOI:10.13705/j.issn.1671-6825.2025.02.042
缺氧对食管鳞状细胞癌细胞HIF-2α表达及迁移和侵袭能力的影响
Effects of hypoxia on expression of HIF-2α and migration,invasion abili-ties of esophageal squamous cell carcinoma cells
摘要
Abstract
Aim:To investigate effects of hypoxia on expression of hypoxia-inducible factor-2α(HIF-2α)and migra-tion,invasion abilities of esophageal squamous cell carcinoma(ESCC)cells.Methods:RT-qPCR and immunohistochemistry were used to detect the expression of HIF-2α in cancerous tissues and paracancerous normal esophageal tissues of 30 ESCC patients,esophageal normal epithelial cells(HEEC)and ESCC cells(KYSE150 and EC109).A cell culture hypoxic envi-ronment was constructed to detect the effect of hypoxia on the expression of HIF-2α in ESCC cells.Scratch and Transwell assays were used to detect the effects of hypoxia,knockdown of HIF-2α and knockdown of HIF-2α during hypoxia on ESCC cells'function.Results:The expression of HIF-2α elevated in ESCC tissues compared with paracancerous normal esophage-al tissues,and ESCC cells compared with HEEC(P＜0.001).Hypoxia promoted the expression of HIF-2α,migration and invasion abilities of ESCC cells;knockdown of HIF-2α inhibited the expression of HIF-2α,migration and invasion abilities;hypoxia and knockdown of HIF-2α had an interactive effect(P＜0.05).Conclusion:Hypoxia could regulate the progres-sion of ESCC by modulating the expression of HIF-2α in ESCC cells.关键词
食管鳞状细胞癌/缺氧/HIF-2α/迁移/侵袭Key words
esophageal squamous cell carcinoma/hypoxia/HIF-2α/migration/invasion分类
医药卫生引用本文复制引用
陈希贤,马素好,陈双双,李萍,杨莹,肖楠,孙莉,刘红春..缺氧对食管鳞状细胞癌细胞HIF-2α表达及迁移和侵袭能力的影响[J].郑州大学学报（医学版）,2025,60(4):480-484,5.基金项目
河南省自然科学基金项目(202300410464) （202300410464）
河南省科技攻关项目(252102311076,252102310080) （252102311076,252102310080）
