湖南农业大学学报（自然科学版）2025，Vol.51Issue(3)：33-41,9.DOI:10.13331/j.cnki.jhau.2025.03.005
罗汉果SgTCP6基因克隆、生物信息学分析及转录激活研究
Cloning,bioinformatics analysis and transcriptional activation analysis of SgTCP6 from Siraitia grosvenorii
摘要
Abstract
Based on the genome and transcriptome information of Siraitia grosvenorii,the TCP transcription factor family SgTCP6 gene was screened and analyzed by bioinformatics,and whether it was involved in the regulation of the key enzyme gene SgUGT94 in the synthesis of mogroside was verified by yeast one-hybrid.The result showed that SgTCP6 gene CDS was 618 bp in length,encoded 205 amino acid residues,and had a relative molecular mass of 21 942.It was subcellularly localized in the nucleus and was an unstable hydrophilic protein without a signal peptide or transmembrane region,thus making it a non-secretory protein.Multiple sequence alignment results showed that the gene was closely related to TCP in Momordica charantia.The co-expression analysis results of genes in Siraitia grosvenorii fruits at different stages showed that there was a significant correlation between the expression of SgTCP6 and SgUGT94.The analysis results of the 2 000 bp promoter upstream of SgUGT94 revealed that this region contained four TCP binding sites.The yeast one-hybrid results showed that SgTCP6 might be involved in the regulation of the key enzyme gene SgUGT94 in the synthesis of mogroside V.关键词
罗汉果/TCP转录因子/生物信息学/酵母单杂交/葡萄糖基转移酶Key words
Siraitia grosvenorii/transcription factor TCP/bioinformatics/yeast one-hybrid/glucosyltransferase分类
农业科技引用本文复制引用
林孝东,唐其,莫长明,穆德添,赵欢,黄华学,邵瑛瑛,陈文强,刘代,梁任繁..罗汉果SgTCP6基因克隆、生物信息学分析及转录激活研究[J].湖南农业大学学报（自然科学版）,2025,51(3):33-41,9.基金项目
国家重点研发计划项目(2023YFD1200200) （2023YFD1200200）
国家自然科学基金项目(32270398,U20A2004,82173915) （32270398,U20A2004,82173915）
湖南省重点研发项目(2022NK2004) （2022NK2004）
