郑州大学学报（医学版）2025，Vol.60Issue(4)：484-487,4.DOI:10.13705/j.issn.1671-6825.2024.12.089
中性粒细胞源性外泌体在乳腺癌细胞侵袭、转移中的作用
Effect of neutrophil-derived exosomes on invasive and metastasis of breast cancer cells
摘要
Abstract
Aim:To explore the effect of neutrophil-derived exosomes(N-Ex)on the invasion and metastasis of breast cancer cells.Methods:The venous blood from the healthy volunteers were collected,and neutrophils were separated.N-Ex was extracted by differential centrifugation,and was to detect the expressions of CD9,CD63 and calnexin by Western blot.MDA-MB-231 breast cancer cells were co-cultured with neutrophils(N),N-Ex,and N+exosome inhibitor(GW4869)for 24 hours,respectively,then the invasion and migration abilities of the cells of the 3 groups were detected using Transwell method,and the expressions of EMT-related proteins in the cells were detected using Western blot.Results:The expressions of CD9 and CD63 were high in the N-Ex,while that of calnexin were low.Compared with the N group,the number of migra-ting and invading cells in the N-Ex group increased(P＜0.05),the expression of E-cadherin decreased,and the expressions of N-cadherin,vimentin,Snail and ZEB proteins increased(P＜0.05);compared with the N group,the number of migrating and invading cells in the N+GW4869 group decreased(P＜0.05),the expressions of E-cadherin and Snail increased,and the expressions of N-cadherin,vimentin and ZEB proteins decreased(P＜0.05).Conclusion:Neutrophils could promote the invasion and migration abilities of breast cancer cells by secreting N-Ex and participating in EMT.关键词
中性粒细胞/外泌体/乳腺癌/迁移/侵袭Key words
neutrophil/exosome/breast cancer/migration/invasion分类
医药卫生引用本文复制引用
张哲,罗怡欣,陈卓,徐国正,赵婷婷,王泳翕,谢晓云,王芳..中性粒细胞源性外泌体在乳腺癌细胞侵袭、转移中的作用[J].郑州大学学报（医学版）,2025,60(4):484-487,4.基金项目
白求恩卓越医学研究基金项目(2023-YJ-153-J-019) （2023-YJ-153-J-019）
