郑州大学学报（医学版）2025，Vol.60Issue(4)：488-492,5.DOI:10.13705/j.issn.1671-6825.2024.09.039
益气养阴活血方对糖尿病肾病大鼠肾功能及SIRT3/SOD2/GPX4通路、铁死亡的影响
Effects of Yiqi Yangyin Huoxue recipe on renal function,SIRT3/SOD2/GPX4 pathway and ferroptosis in rats with diabetic nephropathy
摘要
Abstract
Aim:To explore the effects and possible mechanism of Yiqi Yangyin Huoxue recipe on renal function in rats with diabetic nephropathy(DN).Methods:Totally 42 rats were randomly allocated into normal control group(n=6)and the modeling group(n=36),and the rats in the modeling group were fed with high-fat and high-sugar chow and com-bined with a single intraperitoneal injection of streptozotocin to establish a DN model.The successfully constructed model rats(n=32)were allocated into the model group,the valsartan group(the positive control group),the low dose group of Yiqi Yangyin Huoxue recipe(the low-dose group),and the high-dose group,with 8 rats in each group.The rats in the latter 2 groups were administered with the Chinese traditional medicine at 11.0 and 22.0 g/kg respectively,and the gavage lasted for 6 weeks.After 24 hour urine and blood were collected,the rats were executed,and kidney tissues were taken.The deter-mination of 24 hour total urinary protein(24 h-UTP),serum creatinine(SCr),glucose(GLU),triglycerides(TG),albumin(ALB)by AU5800 Auto-biochemical Analyzer,serum alanine aminotransferase(ALT)was determined by the rate method,and the serum malondialdehyde(MDA)was detected by ELISA.The renal histopathological changes were observed by HE staining.The mRNA and protein expressions of renal tissue silent information regulator 3(SIRT3),superoxide dismutase 2(SOD2),glutathione peroxidase 4(GPX4),and solute carrier family 7 member 11(SLC7A11)were detected by real-time fluorescence quantitative PCR and Western blot.Results:Compared with the normal control group,rats in the model group had severe kidney pathological damage,higher 24 h-UTP,SCr,GLU,TG,ALT and MDA,and lower ALB,lower kidney SIRT3,SOD2,SLC7A11,and GPX4 mRNA and protein expression levels(P＜0.05);compared with the model group,the pathological damage of the kidney was alleviated,the above indexes in the low and high dose groups were improved(P＜0.05),and there was no significant difference in the improvements between the 2 groups(P＞0.05).Conclusion:Yiqi Yangyin Huoxue recipe may inhibit oxidative stress and ferroptosis by activating SIRT3/SOD2/GPX4 signaling pathway,thus reduce renal damage in rats with DN.关键词
益气养阴活血方/糖尿病肾病/SIRT3/SOD2/GPX4/氧化应激/铁死亡/大鼠Key words
Yiqi Yangyin Huoxue recipe/diabetic nephropathy/SIRT3/SOD2/GPX4/oxidative stress/ferroptosis/rat分类
医药卫生引用本文复制引用
季晗舒,任献青,王墨,宋纯东,王旭,樊艳敏,方虹,姜浩然,卜继常,宋珂,陈晨晨..益气养阴活血方对糖尿病肾病大鼠肾功能及SIRT3/SOD2/GPX4通路、铁死亡的影响[J].郑州大学学报（医学版）,2025,60(4):488-492,5.基金项目
国家自然科学基金项目(82074493) （82074493）
河南省卫健委国家中医临床研究基地科研专项(2022JDZX003) （2022JDZX003）
河南省中医药科学研究重大专项(2023ZYZD02) （2023ZYZD02）
河南省中医药学科领军人才项目[豫卫中医函(2021)8号] （2021）
河南省"双一流"创建工程(HSRP-DFCTCM-2023-3-07) （HSRP-DFCTCM-2023-3-07）
