湖南农业大学学报（自然科学版）2025，Vol.51Issue(3)：55-62,8.DOI:10.13331/j.cnki.jhau.2025.03.008
一株兔源A型产气荚膜梭菌的分离鉴定及其生物学特性和药物敏感性
Isolation,identification,biological characteristics and drug sensitivity of a rabbit derived type A Clostridium perfringens strain
摘要
Abstract
The clinical symptoms of 10 dead rabbits in a large-scale rabbit farm in Hebei Province were observed,and then the rabbits were dissected.The pathogenic bacteria that caused the disease in the rabbit farm were determined by isolation and culture,Gram staining,biochemical test,16S rRNA sequence alignment,toxin typing,determination of growth curve,colony counting,antimicrobial susceptibility test,histopathological observation and animal pathogenicity test,and their biological characteristics and drug sensitivity were studied.The results showed that the biochemical characteristics of the isolated Gram-positive bacilli were consistent with those of Clostridium perfringens;its 16S rRNA sequence was 1 380 bp in length,and its similarity to the sequence of type A Clostridium perfringens(No.MK156683.1),reached 99%.The results of isolate toxin typing showed that this gene only carried the cpa virulence gene.The results of animal pathogenicity test showed that all rabbits in the test group died 24 h after infection.The pathogenic microorganism of the diseased organs of the rabbits and infecting strain were identified as the same strain.The results of the drug sensitivity test showed that the isolated Clostridium perfringens type A was sensitive to 10 drugs:gentamicin,kanamycin,butylcarbamazine,azithromycin,madicillin,ciprofloxacin,norfloxacin,enrofloxacin,oxfloxacin and ceftriaxone.关键词
兔/A型产气荚膜梭菌/菌株分离与鉴定/生物学特性/药物敏感性Key words
rabbit/Clostridium perfringens type A/isolation and identification of strain/biological characteristics/drug sensitivity分类
农业科技引用本文复制引用
王帅帅,刘玉梅,李双军,李梦云,谭世图,张自强..一株兔源A型产气荚膜梭菌的分离鉴定及其生物学特性和药物敏感性[J].湖南农业大学学报（自然科学版）,2025,51(3):55-62,8.基金项目
河南省产业科技特派员服务团项目(2020LYCYTPY03) （2020LYCYTPY03）
家兔疫病检测关键技术研究及其产品的研发项目(20220084) （20220084）
家兔病原性腹泻检测技术开发和应用项目(20230158) （20230158）
