湖南农业大学学报（自然科学版）2025，Vol.51Issue(3)：79-88,10.DOI:10.13331/j.cnki.jhau.2025.03.011
不同碳源对金属矿山废弃地土壤微生物群落及修复效应的影响
Effects of different carbon sources on soil microbial community and remediation efficacy in metal mine wasteland
摘要
Abstract
In this study,three common organic wastes,sawdust,chicken manure and silkworm manure were selected as carbon sources and combined with lime and microbial agents for the direct vegetation technology and served to the ecological restoration in metal mine wasteland in Guangdong Province.The three carbon sources were investigated for their remediation efficacies and their impacts on soil microbial community were assessed throughout the restoration process.The results showed that after the three carbon sources restoration,the soil pH increased from 3.27 to 7.19-8.31,and the mass fractions of total organic carbon(except for the samples with half a year of sawdust restoration and one year of chicken manure restoration)and total phosphorus increased significantly,while the mass fractions of DTPA-extractable Cu,Zn,Fe and Pb decreased significantly after half a year of restoration.Among the three carbon sources,chicken manure had the most pronounced effect on enhancing soil nutrients during the initial phase,and silkworm manure had the best effect on reducing soil mass fractions of DTPA-extractable Cu,Zn,Mn,and Fe,while chicken manure exhibited the most substantial suppression of DTPA-extractable Pb.The soil microbial α diversity significantly increased under all three carbon sources after remediation.The types of carbon sources significantly affected the composition of soil microbial communities during the restoration process,and the soil microbial communities showed obvious succession trajectories with the continuous ecological restoration.The relative abundance of functional microbes such as Luteimonas,Bacillus,Devosia and Nitrolancea,increased to promote vegetation growth and nutrient accumulation.During the ecological restoration processes,the environmental factors including pH,net acid production(NAG),NAG-pH,and the mass fractions of total phosphorus,Fe,DTPA-extractable Fe and DTPA-extractable Cu were the main driving factors affecting soil microbial community composition.关键词
金属矿山废弃地/土壤微生物/木屑/鸡粪/蚕粪/碳源/生态修复Key words
metal mine wasteland/soil microbe/sawdust/chicken manure/silkworm manure/carbon source/ecological restoration分类
资源环境引用本文复制引用
高佳,李奇洳,杨富淋,汪华安,何柳,杨春雨,曹百川,邹奇,刘伟..不同碳源对金属矿山废弃地土壤微生物群落及修复效应的影响[J].湖南农业大学学报（自然科学版）,2025,51(3):79-88,10.基金项目
中电工程重大科技专项(DG3-P01-2022) （DG3-P01-2022）
中国能建重大科技专项(CEEC2022-KJZX-02、CEEC2021-KJZX-01) （CEEC2022-KJZX-02、CEEC2021-KJZX-01）
国家自然科学基金项目(42007297) （42007297）
广东省基础与应用基础研究基金项目(2019A1515110345、2021A1515011497) （2019A1515110345、2021A1515011497）
