家畜生态学报2025，Vol.46Issue(6)：16-20,5.DOI:10.3969/j.issn.1673-1182.2025.06.003
甘肃滩羊体线粒体DNA D-loop区遗传多样性及系统进化分析
Genetic Diversity and Phylogenetic Analysis of Mitochondrial DNA D-loop Region in Gansu Tan sheep
摘要
Abstract
In order to investigate the genetic diversity and matrilineal origin of Tan sheep in Gansu province,the study focused on Tan sheep from Huanxian,Jingtai and Jingyuan counties in Gansu Prov-ince.The mtDNA D-loop region sequences of the Tan sheep were obtained through PCR amplification and direct sequencing.The analysis included genetic diversity,genetic structure and the construction of a phy-logenetic tree.The results showed that a total of 7 different mtDNA D-loop sequences of varying lengths(ranging from 1 107 to 1 183 bp,with 1 182 bp being the most common)were identified among the three Tan sheep populations.The average contents of A,G,T,C,A+T and C+G in the three populations were 33.24％,14.29％,29.56％,22.88％,62.87％and 37.18％,respectively,with A+T significantly higher than that of C+G.A total of 154 mutation sites and 33 haplotypes were identified,with an average haplotype diversity(Hd)of 0.977 and nucleotide diversity(Pi)of 0.035 for the mtDNA D-loop sequences across the three Tan sheep populations.Phylogenetic analysis revealed that the 41 Tan sheep sampled could be divided into three branches,suggesting the existence of three maternal lineages among Gansu Tan sheep.The study concludes that Gansu Tan sheep exhibit rich genetic diversity,genetic stability,and three distinct maternal origins.关键词
滩羊/mtDNA D-loop/遗传多样性/系统进化Key words
Tan sheep/mtDNA D-loop/genetic diversity/systematic evolutions分类
农业科技引用本文复制引用
王燕燕,王珂,徐建峰,石福岳,郭海龙,高登伟,顾玲荣,王胜明..甘肃滩羊体线粒体DNA D-loop区遗传多样性及系统进化分析[J].家畜生态学报,2025,46(6):16-20,5.基金项目
中央引导地方科技发展资金项目(24ZYQL003) （24ZYQL003）
甘肃省农业农村厅科技创新项目(GNKJ-2020-6) （GNKJ-2020-6）
