家畜生态学报2025，Vol.46Issue(6)：21-28,8.DOI:10.3969/j.issn.1673-1182.2025.06.004
牦牛和黄牛胎牛组织转录组比较分析
Comparative Analysis of Transcriptome of Yak and Cattle Fetuses
摘要
Abstract
In order to elucidate the adaptation mechanisms of different tissues of yak and cattle fetuses to the high-altitude environment at the transcriptome level,the fetal muscle and brain tissues of three Qinghai plateau yaks and three Chaidamu cattle were selected for transcriptome analysis.The homologous protein coding genes of these two species were identified with the ENSEMBL database.DESeq2 was used to screen out differentially expressed homologous genes,and then the GO and KEGG enrichment analysis was performed to explore the regulatory mechanism of fetal adaptation to plateau hypoxia.The results showed that the high-expressed differential homologous genes in yak and cattle fetal tissues were signifi-cantly enriched in the HIF-1 hypoxia signaling pathway(P＜0.05),and the expression level of HIF1A,EPAS1 and EGLN1 genes,which related to hypoxia adaptation,in cattle fetal tissue is 10 to 100 times higher than that of yak.These results demonstrate that the expression of hypoxia-related genes in yak fetal tissue might be related to its adaptation to living on the plateau,while the cattle fetal tissue responds to the adaptive changes in the plateau hypoxic environment by inducing a large amount highly expressed hy-poxia gene.These results provide the basic data for in-depth study of the hypoxic adaptation mechanism of yak and cattle fetuses.关键词
高海拔/低氧/牦牛/黄牛/转录组Key words
high altitude/hypoxia/yak/cattle/transcriptome分类
农业科技引用本文复制引用
李文莹,朱小贝,张家强,孙乐,任刚,刘俊霞..牦牛和黄牛胎牛组织转录组比较分析[J].家畜生态学报,2025,46(6):21-28,8.基金项目
多能性肌肉卫星细胞细胞系的建立(K3031222085) （K3031222085）
西北农林科技大学高层次引进人才科研启动经费(Z1013023005) （Z1013023005）
