郑州大学学报（医学版）2025，Vol.60Issue(4)：569-572,4.DOI:10.13705/j.issn.1671-6825.2024.09.060
丹皮酚对癫痫大鼠的治疗作用及miRNA-34a/Bcl-2通路的影响
Effects of paeonol on rats with epilepsy and miRNA-34a/Bcl-2 pathway
摘要
Abstract
Aim:To observe the efficacy of paeonol on rats with epilepsy and the possible mechanism.Methods:Thirty male Wistar rats were randomly allocated into 3 groups using simple random sampling,with 10 rats in each group.The rats in normal control group,the epilepsy group and the paeonol group were respectively intraperitoneally injected with normal saline,pentylenetetrazole at a dose of 35 mg/kg,and paeonol at a dose of 40 mg/kg 20 minutes before the injection of penty-lenetetrazole,once a day for 4 weeks.Cell apoptosis in the hippocampal tissue was observed by TUNEL staining.The expres-sions of apoptosis-related proteins Bcl-2,Bax,and cytochrome-C in the hippocampal tissue was detected by Western blot.The activities of superoxide dismutase(SOD)and catalase(CAT),the content of malondialdehyde(MDA),and the total an-tioxidant capacity(T-AOC)in the hippocampal tissue were measured.The expression of miRNA-34a in the hippocampal tis-sue was detected by fluorescence quantitative PCR.Results:Compared with normal control group,the neuronal apoptosis rate increased,the expression levels of Bax,cytochrome-C proteins and miRNA-34a increased,the expression level of Bcl-2 protein decreased,the MDA content increased,and the activities of SOD and CAT and T-AOC decreased in the epilepsy group[(5.74±0.18)vs(1.01±0.31)for miRNA-34a,P＜0.05].Compared with the epilepsy group,all the above indi-cators in the paeonol group were improved[miRNA-34a:(2.85±0.25),P＜0.05].Conclusion:Paeonol has therapeutic effect on epileptic rats,which may be achieved through the mitochondrial oxidative stress pathway mediated by miRNA-34a/Bcl-2 and inhibiting the neuronal apoptosis in the hippocampal tissue.关键词
丹皮酚/微核糖核酸-34a/癫痫/氧化应激/细胞凋亡/大鼠Key words
paeonol/miRNA-34a/epilepsy/oxidative stress/cell apoptosis/rat分类
医药卫生引用本文复制引用
顿圆圆,扈清云,冯燕,朱金玲..丹皮酚对癫痫大鼠的治疗作用及miRNA-34a/Bcl-2通路的影响[J].郑州大学学报（医学版）,2025,60(4):569-572,4.基金项目
黑龙江省教育厅基本科研业务费基础研究项目(2020-KYYWF-0278) （2020-KYYWF-0278）
