中国牛业科学2025，Vol.51Issue(3)：11-19,9.DOI:10.26951/j.cnki.ccs.2025.03.002
水牛MFSD2A基因CDS序列克隆及生物信息学分析
Cloning and Bioinformatics Analysis of CDS Sequence of Buffalo MFSD2A Gene
摘要
Abstract
In this study,bioinformatics method was used to clone and analyze the coding sequence of the major facilitator superfamily domain containing 2a(MFSD2A)gene.The complete coding sequence of buffalo MFSD2A gene was successfully cloned from buffalo MFSD2A predictive gene sequence(GenBank accession number:XM_006061915.4).The sequence is 1 572 bp and encodes 523 amino acids.The molecular formula is C2669H4150N652O725S23,molecular weight is 57.71 ku,the half-life is 30 h,extinction coefficient is 71 445,aliphatic index is 105.56,PI is 8.2,instability index is 34.78,and grand average of hydropathicity is 0.462,indicating that buffalo MFSD2A belongs to alkaline stable hydrophobic protein.The analysis results of secondary and tertiary structures showed that buffalo MFSD2A protein was mainly composed of alpha helix and random coil,in which alpha helix accounted for 46.65％,beta turn accounted for 4.59％,extended strand accounted for 16.25％,random coil accounted for 32.5％.The subcellular localization results showed that the proportion of buffalo MFSD2A located in plasma membrane,endoplasmic reticulum,nucleus and mitochondria was 60.9％,30.4％,4.3％and 4.4％,respectively,indicating that buffalo MFSD2A was mainly distributed in the plasma membrane.The prediction analysis of transmembrane structure and signal peptide showed that the protein had 10 transmembrane domains and no signal peptide,so the protein belonged to transmembrane protein and did not belong to secretory protein.The results of phosphorylation site predic-tion showed that there were 48 phosphorylation sites in buffalo MFSD2A protein,including 22 serine phos-phorylation sites,21 threonine phosphorylation sites and 5 tyrosine phosphorylation sites.Glycosylation site prediction results showed that there was one potential N-glycosylation site and 77 O-glycosylation sites in buffalo MFSD2A protein.The results of homology analysis showed that the homology of buffalo MFSD2A with cattle,goat,pigs,camels domestic dogs and humans was 98.7％,98.5％,94.0％,93.5％,91.0％and 87.0％,respectively,indicating that MFSD2A was highly conservative.关键词
水牛/主要促进因子超家族成员2a(MFSD2A)/基因克隆/生物信息学Key words
buffalo/major facilitator superfamily domain containing 2a(MFSD2A)/gene cloning/bioinformatics分类
农业科技引用本文复制引用
梁莎莎,覃广胜,于农淇,鄢胜飞,黄荣春,潘伟军,李厅厅,卢瑛,黄健,谭正准..水牛MFSD2A基因CDS序列克隆及生物信息学分析[J].中国牛业科学,2025,51(3):11-19,9.基金项目
广西重大科技专项(桂科AA22068099-2,桂科AA22068099-9) （桂科AA22068099-2,桂科AA22068099-9）
"带土移植"人才引育计划(桂科AA23026004) （桂科AA23026004）
国家现代农业产业技术体系广西奶水牛产业创新团队项目(nycytxgxcxtd-2021-21-01) （nycytxgxcxtd-2021-21-01）
