家畜生态学报2025，Vol.46Issue(6)：63-68,6.DOI:10.3969/j.issn.1673-1182.2025.06.010
稀释液及解冻方法对驴精液冷冻效果的影响
Effects of Different Diluents and Thawing Methods on Cryopreservation of Donkey Semen
摘要
Abstract
To investigate the effects of different diluents and thawing methods on the cryopreservation of donkey semen,this experiment utilized glycerol as an cryoprotectant to compare the effects of freezing diluents containing different sugars on the cryopreservation of donkey semen,and selected a group with better results.Subsequently,in this diluent,various antioxidants including Resveratrol(B),Chlorogenic acid(L),Procyanidins from grape seed(P),and their combination were added,and the effects of different antioxidants on the cryopreservation donkey semen were compared.Finally,the selected diluent containing the best combination of sugars and antioxidants was used to cryopreserve donkey semen,and three differ-ent thawing methods were compared for their effects on semen quality.The results showed that after cryo-preservation with the"glucose+lactose"diluent,sperm motility rate,motility,plasma membrane integ-rity and motion parameters were significantly higher than those in the"glucose"and"glucose+sucrose+lactose"diluent group(P＜0.05).Similarly,the above sperm evaluation indicators of the antioxidant P group were significantly higher than those of the BL,LP,and BLP groups(P＜0.05),with no significant difference observed between the B,L,and control groups,but their values were higher.The SOD activity in the P,L and BLP groups was significantly higher than that in the control(P＜0.05).The CAT activity of BL and BP groups was also significantly higher than that of the control(P＜0.05).After thawing the semen at 62 ℃ for 8 seconds,the sperm motility rate,motility,plasma membrane integrity,VCL and VAP were significantly higher than those at 38 ℃ for 30 seconds(P＜0.05).Therefore,the addition of 0.06 g/L proanthocyanidins from grape seeds to"glucose+lactose"diluent can enhance the cryopreserva-tion effect of donkey semen,and the high temperature rapid thawing method at 62 ℃ for 8 seconds is suit-able for thawing frozen donkey semen.关键词
新疆驴/精液稀释液/冷冻保护剂/解冻方法Key words
Xinjiang donkey/semen diluent/frozen protectants/thawing method分类
农业科技引用本文复制引用
王志龙,王璐,郭旭川,张强,曾维斌..稀释液及解冻方法对驴精液冷冻效果的影响[J].家畜生态学报,2025,46(6):63-68,6.基金项目
石河子大学创新发展专项(CXFZ202007) （CXFZ202007）
