中国牛业科学2025，Vol.51Issue(3)：30-36,7.DOI:10.26951/j.cnki.ccs.2025.03.005
腐熟牛粪中耐高温高效纤维素分解菌的筛选及其复合菌系的降解效果
Screening of Thermotolerant and High-Efficiency Cellulose-Decomposing Bacteria from Composted Cattle Manure and Degradation Efficiency of Composite Bacterial Consortia
摘要
Abstract
In this study,high-efficiency cellulose decomposing bacterium were isolated from naturally fermented and decomposed cattle manure by cellulose sodium plate method and Congo red staining me-thod,and the strains were identified by 16SrDNA molecular biology technology.Carboxymethyl cellulase activity was used as the screening index,and the compositing strain was constructed based on the filter pa-per enzyme activity test and antagonistic experiment,and its degradation effect on the stick of enoki mush-room was evaluated.The results showed that two strains with high cellulase activity were identified as Pseudoxanthomonas taiwanensis and Bacillus.The compound strains were compounded in equal propor-tions by the two strains,and the carboxymethyl cellulase activity was 25.22 U/mL,and the filter paper en-zyme activity was 14.95 U/mL.The composite strains showed good degradation ability to Enoki mushroom sticks,with a degradation rate of 47.47％.关键词
纤维素分解菌/菌株筛选/牛粪/降解效果Key words
cellulose decomposing bacterium/strain screening/cattle manure activity/degradation ef-ficiency分类
农业科技引用本文复制引用
祝梦柳,王佳璐,宋月,丁鑫,洪晓青,于洋,祝贺,李波..腐熟牛粪中耐高温高效纤维素分解菌的筛选及其复合菌系的降解效果[J].中国牛业科学,2025,51(3):30-36,7.基金项目
山东省科技型中小企业创新能力提升工程项目(2023TSGC0976) （2023TSGC0976）
山东省重点研发计划(乡村振兴科技创新提振行动计划(2023TZXD046) （乡村振兴科技创新提振行动计划(2023TZXD046）
山东农业工程学院横向科研项目(sgyhx2023-37) （sgyhx2023-37）
