中国当代医药2025，Vol.32Issue(17)：4-9,27,7.DOI:10.3969/j.issn.1674-4721.2025.17.01
人参皂苷Rb1调控血管平滑肌细胞表型转换的机制
Mechanism of ginsenoside Rb1 in regulating phenotypic switching of vas-cular smooth muscle cells
摘要
Abstract
Objective To explore the protective effect and mechanism of ginsenoside Rb1 in the phenotype transformation of vascular smooth muscle cells(VSMCs).Methods VSMCs cultured and passages to between 3rd and 6th generation were divided into Con+PBS group,Con+Rb1 group,Ang Ⅱ group,Ang Ⅱ+Rb1 group,AV-FoxO3a+Ang Ⅱ group,si-Fox-O3a+Ang Ⅱ group,AV-FoxO3a+Ang Ⅱ+Rb1 group,si-FoxO3a+Ang Ⅱ+Rb1 group,each group was repeated 4 times,and each group was starved for 24 h before treatment,and Rb1 was added 6 h before angiotensin Ⅱ(Ang Ⅱ)treatment.AV-FoxO3a,si-FoxO3a were transfected for 24 h and then starved for 24 h.Rb1 was added 6 h before Ang Ⅱ treatment.Scratch assay was used to detect the migratory ability of VSMCs,protein immunoblotting(Western blot)was used to detect forkhead box protein O3a(FoxO3a),phosphorylated forkhead box protein O3a(p-FoxO3a),α-smooth muscle actin(α-SMA),matrix metalloproteinase 2(MMP2),calmodulin 1(CNN1),smooth muscle 22 α(SM22 α)protein expression levels.Results The results of scratch experiment showed that the migratory ability of VSMCs in Ang Ⅱ+Rb1 group was significantly lower than that in Ang Ⅱ group.The re-sults of Western blot assay showed that the expression lev-els of α-SMA,CNN1,SM22 α,and p-FoxO3a in Ang Ⅱ group were lower than those in Con+PBS group,and the differ-ences were statistically significant(P＜0.05).The expression levels of FoxO3a and MMP2 were significantly higher than those in the Con+PBS group,and the differences were statistically significant(P＜0.05).The expression levels of α-SMA,CNN1,SM22 α,and p-FoxO3a in the Ang Ⅱ+Rb1 group were higher than those in the Ang Ⅱ group,and the differences were statistically significant(P＜0.05).The expression levels of FoxO3a and MMP2 were lower than those in the Ang Ⅱ group,the differences were statistically significant(P＜0.05).The α-SMA,CNN1,and SM22 α in the AV-FoxO3a+Ang Ⅱgroup were lower than those in the si-FoxO3a+Ang Ⅱ group,while MMP2 expression levels was higher than that in the si-FoxO3a+Ang Ⅱ group,with statistical significances(P＜0.05).The α-SMA,CNN1,and SM22 α in the AV-Fox-O3a+Ang Ⅱ+Rb1 group and si-FoxO3a+Ang Ⅱ+Rb1 group were higher than those the AV-FoxO3a+Ang Ⅱ group,while the MMP2 expression level was lower than that of the AV-FoxO3a+Ang Ⅱ group,with statistical significances(P＜0.05).Conclusion Rb1 inhibit the phenotype transformation and the expression of FoxO3a in VSMCs induced by Ang Ⅱ.关键词
人参皂苷Rb1/FoxO3a/腹主动脉瘤/血管平滑肌细胞/表型转换Key words
Ginsenoside Rb1/FoxO3a/Abdominal aortic aneurysm/Vascular smooth muscle cell/Phenotypic transforma-tion分类
医药卫生引用本文复制引用
苏照海,曹竣,谢正,陆伟灵..人参皂苷Rb1调控血管平滑肌细胞表型转换的机制[J].中国当代医药,2025,32(17):4-9,27,7.基金项目
江西省中医药管理局科技计划项目(2020A0305) （2020A0305）
江西省赣州市卫生健康委员会市级科研计划项目(GZWJW202402303). （GZWJW202402303）
