海洋科学2025，Vol.49Issue(2)：15-24,10.DOI:10.11759/hykx20240821001
黑指纹海兔Aplysia dactylomela中的萜类化合物及其抗炎和细胞毒活性
Terpenoids with anti-inflammatory and cytotoxicity activities from the sea hare Aplysia dactylomela
摘要
Abstract
This study investigated the chemical components and their biological activities of the sea hare Aplysia dactylomela,which was collected from Qi Lian Yu,Sansha,Hainan.The secondary metabolites of A.dactylomela were isolated and purified through silica gel chromatography,octadecylsilyl,Sephadex LH-20 gel column chroma-tography,and semipreparative high-performance liquid chromatography.Their structures were identified by nuclear magnetic resonance spectroscopy and mass spectrometry data and compared with literature data.Eleven terpenoids were identified from the sea hare A.dactylomela,including palisadin A(1),(-)-aplysistatin(2),5β-acetoxypalisadin A(3),5-acetoxypalisadin B(4),luzodiol(5),pileaterpene C(6),isoaplysin-20(7),isoconcinndiol(8),cis-pachydictyol B(9),dictytriol(10),and brevisnoside A(11).The results of anti-inflammatory and cytotoxicity activity tests showed that compounds 1-4,6,and 10 had different levels of anti-inflammatory activity and no cell toxicity.Specifically,the anti-inflammatory activity of compound 2(with an IC50 value of(3.42±0.25)µmol/L)was more pronounced than the anti-inflammatory activity of positive control quercetin(with an IC50 value of(9.98±1.14)µmol/L).Compounds 1 and 4 had medium cytotoxicity activity on human chronic myeloid leukemia cells(K562),with IC50 values of(27.82±0.06)and(19.99±1.96)µmol/L,respectively.关键词
黑指纹海兔/化学成分/溴化萜类化合物/抗炎活性/细胞毒活性Key words
Aplysia dactylomela/chemical constituent/terpenoid bromide/anti-inflammatory activity/cytotoxicity activity分类
化学化工引用本文复制引用
邓家珍,彭翰阳,曾艳波..黑指纹海兔Aplysia dactylomela中的萜类化合物及其抗炎和细胞毒活性[J].海洋科学,2025,49(2):15-24,10.基金项目
国家自然科学基金(41776093) （41776093）
中国热带农业科学院国家热带农业科学中心科技创新团队项目(CATASCXTD202416)National Natural Science Foundation of China,No.41776093 （CATASCXTD202416）
Chinese Academy of Tropical Agricultural Sciences for Science and Technology Innovation Team of National Tropical Agricultural Science Center,No.CATASCXTD202416 （）
