核农学报2025，Vol.39Issue(8)：1593-1605,中插1,14.DOI:10.11869/j.issn.1000-8551.2025.08.1593
能质子束辐射提高水稻耐盐碱性的多维度分析
Multidimensional Analysis on the Improvement of Salinity Tolerance in Rice by Medium Energy Proton Beam Irradiation
摘要
Abstract
To explore the potential application of medium-energy proton beam irradiation to enhance salinity-alkalinity tolerance in rice,the impacts of medium-energy proton beam irradiation on salinity tolerance in rice were investigated at phenotypic,physiological,biochemical,and genomic levels and salinity tolerant mutants were screened.Results showed that proton irradiation had a greater impact on seed viability than microgravity.Specifically,low doses(≤10 Gy)of proton irradiation promoted seed germination,whereas high doses(≥100 Gy)inhibited it.Rice mutant obtained with 10 Gy proton beam irradiation exhibited increased chlorophyll content and concomitant enhancement in seedling photosynthesis when subjected to salinity stress.Compared to the wild-type(WT),the mutant exhibited significantly higher superoxide dismutase(SOD)and catalase(CAT)activities along with significantly reduced malondialdehyde(MDA)content,indicating enhanced antioxidant capacity.Whole-genome sequencing(WGS)analysis demonstrated that 53.5％of proton beam-induced single-base substitutions(SBSs)and insertions/deletions(InDels)occurred in intergenic regions.SBSs were the most abundant variant type,accounting for 80.4％of all mutations.Single-base transitions constituted the predominant type among all observed SBSs,with an average transition/transversion ratio of 3.0.Over half of the InDels were short-fragment mutations(1-2 bp).KEGG pathway enrichment analysis suggested that rice may counteracts salinity-alkalinity stress primarily through secondary metabolites biosynthesis,plant hormone signal transduction,phenylpropanoid biosynthesis,and glutathione metabolism.This study provides theoretical guidance for employing medium-energy proton irradiation to improve salinity tolerance in rice and for elucidating the underlying mutagenic mechanisms.关键词
质子束辐射/全基因组重测序/盐碱胁迫/抗氧化保护系统/水稻Key words
beam proton radiation/whole genome resequencing/saline and alkaline stress/antioxidant protection systems/rice引用本文复制引用
赵哲,罗增铜,孙凯,田梦晴,程梓轩,代伽娴,郭涛,肖武名..能质子束辐射提高水稻耐盐碱性的多维度分析[J].核农学报,2025,39(8):1593-1605,中插1,14.基金项目
中国原子能研究院抗辐照创新基金项目(KFZC2021010401),广东省级乡村振兴战略专项资金种业振兴项目(2022-NPY-00-016) （KFZC2021010401）
