江西农业大学学报2025，Vol.47Issue(3)：569-586,18.DOI:10.3724/aauj.2025049
水稻穗发芽抗性鉴定方法优化及批量筛选
Optimization of identification methods and mass screening for pre-harvest sprouting resistance in rice
摘要
Abstract
[Objective]This study aims to establish a scientific and convenient evaluation system for identifying pre-harvest sprouting(PHS)resistance in rice and screening PHS-resistant germplasm resources,thereby providing technical support for breeding PHS-resistant varieties.[Method]16 rice experimental lines were used as research material to optimize the evaluation methodology and sampling timing for PHS-resistance.Subsequently,the optimized protocol was applied to conduct artificial panicle sprouting tests on 360 rice accessions grown under normal seasonal conditions in Nanjing during 2023-2024.Cluster analysis was performed to assess PHS resistance levels,followed by correlation analyses with heading date characteristics and field sprouting performance.[Result]The study demonstrated that:(1)Evaluation of 16 rice test lines sampled at 20-50 days after flowering(DAF)revealed that panicles collected at 35 DAF exhibited stable relative water content while maintaining consistent maturity,thereby better differentiating PHS-resistance among materials.Compared with the grain germination method,the whole-panicle germination method reflected more effectively varietal differences in PHS-resistance with improved labor efficiency and time efficiency.(2)The average germination rates from whole-panicle sprouting tests of the 360 rice accessions followed a normal distribution,showing similar variation trends between the two years.Significant differences(P<0.05)in pre-harvest sprouting(PHS)rates among varieties were observed on day 4,and the differences diminished after day 6.(3)The daily average germination rates from the artificial whole-panicle sprouting tests conducted in 2023 and 2024 were normalized separately and subsequently analyzed using K-means clustering.Based on this analysis,the 360 tested rice accessions were classified into three pre-harvest sprouting(PHS)resistance categories:highly PHS-resistant(Cluster I),moderately PHS-resistant(Cluster II),and PHS-susceptible(Cluster III).(4)Statistical analysis of the correlation between heading dates and PHS test results demonstrated that rice accessions flowering from August 5 to 15 under conventional cultivation conditions in Nanjing exhibited significantly elevated panicle sprouting rates(P<0.05),suggesting their inherent susceptibility to PSH.(5)By comparing the artificial PHS test results from 2023-2024 with the actual field PHS performance in the corresponding years,the following PHS resistance evaluation criteria was established as following:For the high spike germination-resistant type,the germination rate was less than 15%on the 4th day and less than 25%on the 6th day in the whole spike germination test;for the spike germination-prone type,the germination rate was more than 20%on the 4th day and more than 50%on the 6th day in the whole spike germination test.[Conclusion]For the whole panicles collected at 35 DAF,the artificial whole-panicle germination test can be used to classify rice PHS resistance types based on the germination rates recorded on day 4 and day 6.This method provides a technical reference for breeding PHS-resistant rice varieties.关键词
水稻/穗发芽/批量筛选/聚类分析/抗性鉴定Key words
rice/pre-harvest sprouting/mass screening/cluster analysis/resistance identification分类
农业科技引用本文复制引用
李静,李雨欣,赵洁,黄奕,李霞,谢寅峰,杨杰..水稻穗发芽抗性鉴定方法优化及批量筛选[J].江西农业大学学报,2025,47(3):569-586,18.基金项目
国家自然科学基金项目(31571585)、江苏省农业科技自主创新资金项目[CX(23)1001]、江苏省重点研发计划(现代农业)(BE2021359)、江苏省种业创新"揭榜挂帅"项目[JBGS(2021)038、JBGS(2021)039]和国家重点研发计划(2023YFE01117000)Project supported by the National Natural Science Foundation of China(31571585),Jiangsu Province Agricultural Science and Technology Independent Innovation Fund Project[CX(23)1001],the Key Research and Development Program of Jiangsu Province(Modern Agriculture)(BE2021359),the"Jiangsu Province Seed Industry Innovation"Project[JBGS(2021)038 and JBGS(2021)039)and the National Key R&D Program of China(2023YFE01117000) 生物育种钟山实验室(BM2022008-03,ZSBBL-KY2023-01,ZSBBL-KY2003-06-1-2,ZSBBL-KY2024-01)同时对本研究给予了资助,谨致谢意! （31571585）
