日用化学工业（中英文）2025，Vol.55Issue(7)：909-919,11.DOI:10.3969/j.issn.2097-2806.2025.07.012
基于双培养体系的化妆品微生物ATP生物荧光增幅法适用性研究
Suitability study of amplified ATP bioluminescence assay for microbial detection of cosmetics based on two cultivation systems
摘要
Abstract
In order to evaluate the suitability of the amplified ATP bioluminescence assay based on two cultivation systems in the rapid screening of cosmetic microorganisms,51 commercially available cosmetic products covering 20 domestic and foreign brands,including different product types,functional claims,action sites,product dosage forms,and user groups,were widely selected for method suitability research with reference to the method suitability test requirements of sterility inspection method and microbial counting method in the Chinese Pharmacopoeia(2020 Edition).And the suitability of the method was evaluated through sample impact test and spiking test results.The results show that 45 of the 51 products have a background fluorescence signal value less than 3-fold the signal value of blank medium,which meet the requirements of the sample impact test.While six products failed the sample impact test.Six representative strains are inoculated in 45 products that meet the requirements of the sample impact test according to the final concentration of 10-100 CFU/g,and are positively detected in 42 products after being cultured in the two cultivation systems.While some strains in three test products do not get positive detection results.In conclusion,the amplified ATP bioluminescence assay based on two cultivation systems has good suitability in the rapid screening of microbial contamination samples in cosmetics,and provides an efficient and reliable new method for microbial safety control in the whole process of cosmetics production.关键词
双培养体系/ATP生物荧光增幅法/化妆品/微生物/适用性Key words
two cultivation systems/amplified ATP bioluminescence assay/cosmetics/microorganism/suitability分类
化学化工引用本文复制引用
刘瑞娜,姜彪,林雅芳,杨丽哲,翟磊,李昆,姚粟..基于双培养体系的化妆品微生物ATP生物荧光增幅法适用性研究[J].日用化学工业（中英文）,2025,55(7):909-919,11.基金项目
横向项目:化妆品微生物ATP生物荧光增幅24h检测方法建立及验证研究 （）
