摘要
Abstract
In this study,a high performance liquid chromatography(HPLC)method was established to simultaneously detect six major flavonoids(naringin,hesperidin,neohesperidin,naringenin,eriocitrin,and tangeretin)in grapefruit extract toothpaste.The mobile phase was methanol and 0.1%formic acid aqueous solution,and the toothpaste extraction solvent was 80%methanol.The results of the methodological investigation show that the precision RSD ranges from 0.93%to 1.42%,the stability RSD ranges from 0.94%to 1.42%,and the repeatability RSD ranges from 1.14%to 1.44%,and all RSDs are less than 2.0%.The six flavonoids show a good linear relationship in the mass concentration range of 1-100 μg/mL,with a correlation coefficient of 0.999 4-0.999 8.The average recovery rate of the six flavonoids ranges from 95.20%to 104.57%,and the RSD ranges from 0.51%to 2.06%.The detection method in this study can accurately detect the content of the six flavonoids in grapefruit extract toothpaste,which has a reference value for the quality control of grapefruit extract toothpaste.关键词
高效液相色谱法/牙膏/葡萄柚提取物/黄酮类物质/同时检测/质量控制Key words
HPLC/toothpaste/grapefruit extract/flavonoids/simultaneous detection/quality control分类
化学化工
