日用化学工业（中英文）2025，Vol.55Issue(7)：943-948,6.DOI:10.3969/j.issn.2097-2806.2025.07.016
高效液相色谱法同时测定口腔护理牙膏中10种季铵盐类杀菌剂含量
Simultaneous determination of 10 quaternary ammonium salt bactericides in oral care toothpaste by high performance liquid chromatography
摘要
Abstract
A high performance liquid chromatography(HPLC)method was established for the simultaneous determination of 10 quaternary ammonium salt compounds(benzalkonium chloride,benzethonium chloride,dequalinium chloride,lauralkonium chloride,methamphetamine chloride,octadecyl dimethyl benzyl ammonium chloride,cetyltrimethylammonium chloride,cetylpyridinium chloride,cetrimide bromide,and cetrimide chloride)in oral care toothpaste.Acetonitrile was used as the extraction solvent,with a mobile phase of 60 mmol/L ammonium acetate buffer solution(pH=6.0)and acetonitrile for gradient elution.The column temperature was 30℃,the flow rate was 1.0 mL/min,and the detection wavelength was 210 nm.The results of the methodological evaluation show that the precision RSD of 10 quaternary ammonium salts is less than 6%.The 10 quaternary ammonium salts show good linear relationship in the mass concentration range of 0.1-500 mg/L,with correlation coefficients of 0.999 4-0.9998 and detection limits of 0.03-0.09 mg/L.The average recovery of 10 quaternary ammonium salts ranges from 96.80%to 105.25%,and the RSD ranges from 0.74%to 5.45%.Using this method,benzalkonium chloride(128.09 mg/kg),dequalinium chloride(329.13 mg/kg)and cetylpyridinium chloride(231.04 mg/kg)are detected in three toothpastes,respectively.This method has good separation effect and high precision,and can be used for the detection of quaternary ammonium salts in oral care toothpastes.关键词
高效液相色谱法/口腔护理牙膏/同时测定/季铵盐类化合物/杀菌剂Key words
high performance liquid chromatography/oral care toothpaste/simultaneous determination/quaternary ammonium salts/bactericide分类
化学化工引用本文复制引用
范丽敏,何美玲,原丽莹,申亚利,谢冰冰,刘培林..高效液相色谱法同时测定口腔护理牙膏中10种季铵盐类杀菌剂含量[J].日用化学工业（中英文）,2025,55(7):943-948,6.基金项目
河南省高校人文社会科学研究一般项目(2025-ZZJH-128) （2025-ZZJH-128）
