上海农业学报2025，Vol.41Issue(3)：22-29,8.DOI:10.15955/j.issn1000-3924.2025.03.03
同源四倍体新材料不结球白菜'绿星'的创制
Creation of a new material of autotetraploid non-heading Chinese cabbage 'Lvxing'
摘要
Abstract
Using the diploid non-heading Chinese cabbage'Lvxing'L033 as the experimental material,the growth point of shoot tip was treated with 0.2％(m/V)colchicine aqueous solution at the cotyledon stage.The autotetraploid was identified by morphological and cytological methods,and the nutritional quality of diploid and tetraploid plants was compared.The results showed that the floral organs,flowers and siliques of tetraploid plants were larger than those of diploid plants,but the plant expansion and leaves tended to become smaller,and there was no significant difference in seed size.The stomata of tetraploid plants became significantly larger and the stomatal density decreased,and the pollen shape changed irregularly.The number of chromosomes and the DNA fluorescence intensity in tetraploid plants were twice that in diploid plants.Compared with the diploid,the content of cellulose,chlorophyll and total free amino acids of autotetraploid was significantly increased by 132.35％,34.66％ and 21.17％,respectively,while the content of nitrate nitrogen,soluble sugar and soluble protein was increased by 0.24％,12.43％ and 1.92％,respectively.Compared with the diploid,the plant height,weight per plant,ten-leaf thickness,leaf length/width,petiole length/width and leaf number of autotetraploid decreased to varying degrees,while the petiole thickness and single leaf weight increased by 9.10％ and 4.54％,respectively,and the net photosynthetic rate increased significantly.In this study,a dwarfed and compact tetraploid'Lvxing'was obtained,and a new non-heading Chinese cabbage germplasm resource was created.It was also proved that colchicine did not absolutely cause the"gigantic"of vegetative organs in the doubling process of plant genome,providing new ideas for the innovation of non-heading Chinese cabbage germplasm resources..关键词
不结球白菜/'绿星'/秋水仙素/四倍体/矮紧型Key words
Non-heading Chinese cabbage/'Lvxing'/Colchicine/Tetraploid/Dwarf分类
农业科技引用本文复制引用
李丹,张彦文,何英子,侯喜林,李英,肖栋,刘同坤..同源四倍体新材料不结球白菜'绿星'的创制[J].上海农业学报,2025,41(3):22-29,8.基金项目
江苏省农业科技自主创新资金[CX(23)3023] （23）
江苏省种业揭榜挂帅项目[JBGS(2021)015] （2021）
三亚市科技创新专项项目(2022KJCX80) （2022KJCX80）
