上海农业学报2025，Vol.41Issue(3)：56-62,7.DOI:10.15955/j.issn1000-3924.2025.03.07
'红颜'草莓茎尖组培成苗技术研究
Propagation technology of tissue culture of stem apex in'Benihoppe'strawberry
摘要
Abstract
In order to optimize and improve the strawberry stem apex tissue culture seedling technology.taking'Benihoppe'strawberry as the test material and stem apex as the explant,based on the MS medium,the optimal developmental state of the collected stem apex,the optimal time for sodium hypochlorite disinfection,the optimal ratio of the propagation and rooting medium to the substrate for seedling growth were investigated,and the flowering and fruiting conditions of the tissue culture seedlings were observed.The results showed that the optimal developmental state for collecting the stem apex of'Benihoppe'strawberries was when the first leaf of the stolon was less than 1/2 unfolded and the second leaf was not fully unfolded.Disinfection was most suitable with alcohol treatment for 30 seconds and 2.5％ sodium hypochlorite treatment for 10 minutes.The optimal propagation medium was MS+6-BA(0.5 mg/L).The optimal rooting medium was 1/2 MS+IBA(0.5 mg/L).The optimal substrate volume ratio for seedling growth was weathered sand∶peat soil∶garden soil=1∶1∶1.After the tissue culture seedlings were transplanted,the flowering and fruiting were normal.This plan could provide a reference for the factory-based seedling cultivation of strawberries.关键词
草莓/茎尖/组织培养/基质Key words
Strawberry/Apex/Tissue culture/Substrate分类
农业科技引用本文复制引用
刘修丽,刘晓荣,滕龙,马跃,刘继恩,王宏光,王志刚..'红颜'草莓茎尖组培成苗技术研究[J].上海农业学报,2025,41(3):56-62,7.基金项目
辽宁省"揭榜挂帅"科技项目(2022JH1/10400016) （2022JH1/10400016）
辽宁省种质创新藏粮于技专项计划(2023JH/10200003) （2023JH/10200003）
辽宁省农村科技特派行动计划(2023JH5/10400049) （2023JH5/10400049）
辽宁省农业科学院基本科研业务费科技服务(2024-XCZX-A1-10) （2024-XCZX-A1-10）
