中国森林病虫2025，Vol.44Issue(4)：1-8,8.DOI:10.19688/j.cnki.issn1671-0886.20250006
RNA干扰分析表皮蛋白基因HcCPG对美国白蛾生长发育的影响
Effects of cuticular protein gene HcCPG on growth and development of Hyphantria cunea using RNA interference analysis
摘要
Abstract
To study the effects of cuticular protein glycine-rich(CPG)gene family from Hyphantria cunea(HcCPG)on growth and development of H.cunea,the gene HcCPG was cloned and its characteristics were analyzed.Real-time fluorescent quantitative polymerase chain reaction technology was employed to detect expression levels of HcCPG gene at different developmental stages and various organs(tissues)of larvae.RNA interference(RNAi)technology was used to study the regulatory effect of HcCPG gene in the growth and development of H.cunea.Results indicated that open reading frame(ORF)of HcCPG gene was 828 bp encoding 275 amino acids.The expression level of HcCPG gene was the highest in the seventh instar larvae and silk glands.After silencing the HcCPG gene,feeding amount and body mass of larvae were significantly decreased,developmental duration was significantly extended,molting became difficult,and mortality rate increased significantly.HcCPG gene plays an important role in the growth,development,and molting processes of H.cunea.关键词
美国白蛾/表皮蛋白/RNA干扰/存活率/生长发育Key words
Hyphantria cunea/cuticular protein/RNA interference/survival rate/growth and development分类
农业科技引用本文复制引用
邱亚丽,王启俐,孙丽丽,曹传旺..RNA干扰分析表皮蛋白基因HcCPG对美国白蛾生长发育的影响[J].中国森林病虫,2025,44(4):1-8,8.基金项目
国家重点研发计划项目"重大林草入侵生物扩张蔓延机制与高效防控技术研究"(2021YFD1400300) （2021YFD1400300）
