中国森林病虫2025，Vol.44Issue(4)：9-15,7.DOI:10.19688/j.cnki.issn1671-0886.20250009
北京古侧柏叶枯病病原真菌鉴定与致病性验证
Pathogenic fungal identification and pathogenicity verification of leaf blight disease of ancient Platycladus orientalis in Beijing
摘要
Abstract
Leaf blight disease of ancient Platycladus orientalis was common in Beijing,severely affecting its survival and ornamental value.The pathogen was isolated from two leaf specimens of leaf blight disease collected from the Changling Tomb and the Summer Palace in Beijing.The pathogen was preliminarily identified by morphological observation and combined with internal transcribed spacer(ITS),glyceraldehyde-3-phosphate dehydrogenase(GAPDH)and translation elongation factor1-alpha(TEF1)multi-gene phylogenetic analysis,and the pathogenicity verification test was carried out.Fungal strains were identified as two species of Bipolaris,namely Bipolaris sorokiniana and Bipolaris setariae.Koch's postulates confirmed that both pathogenic fungi could cause leaf blight disease of P.orientalis.This study is the first to report leaf blight disease of P.orientalis caused by Bipolaris spp.,providing a basis for the diversity of pathogenic fungi causing leaf blight disease and disease management of ancient P.orientalis in Beijing.关键词
古侧柏/叶枯病/病原鉴定/致病性检验Key words
ancient Platycladus orientalis/leaf blight/pathogen identification/pathogenicity test分类
农业科技引用本文复制引用
焦宁,张哲,马静怡,张英..北京古侧柏叶枯病病原真菌鉴定与致病性验证[J].中国森林病虫,2025,44(4):9-15,7.基金项目
国家自然科学基金项目"中国蓝莓病原真菌的物种多样性及其致病性研究"(31971658),"中国假尾孢属(Pseudocercospora)的系统学研究"(31770015)和"中国黑星菌科真菌的系统分类研究"(31370063) （31971658）
国家自然科学基金国际合作与交流项目"中国及南非葡萄腔菌科真菌多样性与分类研究"(31461143028) （31461143028）
