山西医科大学学报2025，Vol.56Issue(6)：636-643,8.DOI:10.13753/j.issn.1007-6611.2025.06.007
麝香保心丸通过抑制TGF-β/Smad/ERK信号通路缓解急性心肌梗死大鼠心肌纤维化
Shexiang Baoxin pill alleviates myocardial fibrosis in rats with acute myocardial infarction by inhibiting TGF-β/Smad/ERK signaling pathway
摘要
Abstract
Objective To explore the effect of Shexiang Baoxin pill(SBP)on myocardial fibrosis progression in rats with acute myo-cardial infarction(AMI)based on the TGF-β/Smad/ERK signaling pathway.Methods Forty-eight male SD rats were randomly di-vided into sham group,model group,low-dose SBP group(L-SBP),and high-dose SBP group(H-SBP).AMI model was induced by li-gation of the left anterior descending coronary artery.After successful modeling,the rats in L-SBP group and H-SBP group were admini-stered orally with SBP for 8 weeks.The cardiac functions of rats in each group were detected by small animal echocardiography.Hema-toxylin-eosin(HE)staining was used to evaluate the myocardial infarction area of rats.ELISA was used to detect the expressions of pro-inflammatory cytokines IL-6 and TNF-α in serum.qRT-PCR was used to measure the transcription levels of myocardial type Ⅰ and Ⅲcollagen fibers.Masson's trichrome staining was performed to analyze collagen fiber expression in myocardial tissue.Western blotting was used to detect the expressions of TGF-β1,p-Smad2/3,and p-ERK1/2 proteins in myocardial tissue.Results Compared with sham group,the heart function decreased(P<0.05),the myocardial infarct size increased(P<0.000 1),serum levels of TNF-α and IL-6 increased(P<0.01),the transcriptional levels of type Ⅰ and type Ⅲ collagen fibers in myocardial tissue increased(P<0.001),the contents of collagen fibers increased significantly(P<0.000 1),and the expressions of TGF-β1,p-Smad2/3,and p-ERK1/2 proteins increased in model group(P<0.05).Compared with model group,the heart function was improved in L-SBP group and H-SBP group(P<0.05),the myocardial infarct size decreased(P<0.001),the levels of inflammatory factors and the degree of myocardial fibrosis decreased significantly(P<0.05),and the expressions of TGF-β1,p-Smad2/3,and p-ERK1/2 proteins decreased(P<0.05).Com-pared with L-SBP group,the heart function was further improved(P<0.05),the myocardial infarct size further decreased(P<0.01),the levels of inflammatory factors and the myocardial fibrosis decreased(P<0.05),and the expressions of TGF-β1,p-Smad2/3,and p-ERK1/2 proteins decreased in H-SBP group(P<0.05).Conclusion SBP can inhibit the systemic inflammatory response in AMI rats by suppressing the TGF-β/Smad/ERK signaling pathway,thereby reversing myocardial fibrosis,promoting repair of damaged myo-cardium,and improving heart function.关键词
麝香保心丸/急性心肌梗死/纤维化/IL-6/TNF-α/心功能/TGF-β/Smad/ERK信号通路Key words
Shexiang Baoxin pill/acute myocardial infarction/fibrosis/IL-6/TNF-α/cardiac function/TGF-β/Smad/ERK signaling pathway分类
医药卫生引用本文复制引用
刘茂林,樊林花,尚小森,卫兵艳..麝香保心丸通过抑制TGF-β/Smad/ERK信号通路缓解急性心肌梗死大鼠心肌纤维化[J].山西医科大学学报,2025,56(6):636-643,8.基金项目
太原市科技计划项目(202264) （202264）
山西省卫生健康委科研项目(2019122) （2019122）
山西省基础研究计划项目青年科学研究项目(202403021212288) （202403021212288）
