实用肝脏病杂志2025，Vol.28Issue(4)：497-500,4.DOI:10.3969/j.issn.1672-5069.2025.04.005
HepG2和HepG2.2.15细胞ENPP2/EZH2表达变化及其对细胞增殖和侵袭力的影响
Ectonucleotide pyrophosphatase/phosphodiesterase 2 and enhancer of Zeste homolog 2 in HepG2 and HepG2.2.15 cells and their impact on cell proliferation and invasiveness
摘要
Abstract
Objective The aim of this experiment was to investigate tumor-promoting elements,e.g.,ectonucleotide pyrophosphatase/phosphodiesterase 2(ENPP2)and enhancer of Zeste homolog 2(EZH2)in HepG2 and HepG2.2.15 cells and their impact on cell proliferation and invasiveness.Methods For inhibition of ENPP2 and EZH2 expression,siRNAs were synthesized,and HepG2,HepG2.2.15,pSM2 plasmid-transfected HepG2(HepG2/pSM2)and siENPP2 or siEZH2-transfected HepG2.2.15 cells were harvested.ENPP2 and EZH2 mRNAs were detected by real-time PCR and their expression was detected by Western blot.Proliferation and invasion of cells were detected by CCK-8 and Trans-well movement.Results ENPP2 and EZH2 mRNA loads in transient HBV-plasmid transfected HepG2 cells or HepG2.2.15 cells with stable HBV infection increased greatly,compared to those in HepG2 cells(P＜0.001),and ENPP2 and EZH2 protein expression also intensified(P＜0.001);proliferation and invasiveness of HepG2.2.15 cells were obviously elevated compared to in HepG2 cells(P＜0.01),while inhibition of ENPP2 or EZH2 expression in HepG2.2.15 cells greatly weakened proliferation and invasiveness of the cells(P＜0.01).Conclusion The intensified expressions of ENPP2 and EZH2 in HepG2 cells and in HepG2.2.15 cells might play a pivotal role in carcinogenesis,which needs further investigation.关键词
HepG2细胞/HepG2.2.15细胞/外核苷酸焦磷酸酶/磷酸二酯酶/Zeste增强子同源物2/细胞增殖/细胞侵袭/体外Key words
HepG2 cells/HepG2.2.15 cells/Ectonucleotide pyrophosphatase/phosphodiesterase 2/Enhancer of Zeste homolog 2/Proliferation/Invasion/In vitro引用本文复制引用
邓万玉,黄子乐,刘泽妍,秦培毓,刘娜..HepG2和HepG2.2.15细胞ENPP2/EZH2表达变化及其对细胞增殖和侵袭力的影响[J].实用肝脏病杂志,2025,28(4):497-500,4.基金项目
国家自然科学基金资助项目(编号:82260603) （编号:82260603）
江西省自然科学基金资助项目(编号:20224BAB206005) （编号:20224BAB206005）
