中国土壤与肥料Issue(5)：79-86,8.DOI:10.11838/sfsc.1673-6257.24530
双季稻田土壤DNRA菌群落丰度与结构对不同施氮的响应
Response of DNRA bacterial abundance and community to different nitrogen applications in paddy soils
摘要
Abstract
Nitrate isomerization and reduction to ammonium(DNRA)is conducive to nitrogen fixation in the soil.The effects of long-term different nitrogen application on microbial abundance and community structure of DNRA process in paddy soil were studied to provide scientific basis for rational fertilization in paddy fields.Four treatments of no nitrogen fertilizer(CK),conventional urea(NPK),controlled-release urea(CRU1)and controlled-release urea with 30%nitrogen reduction(CRU2)were set up,and the abundance and community structure of DNRA bacteria in rice soil were analyzed by fluorescence quantitative PCR and high-throughput sequencing.The results showed that nitrogen fertilization had a certain inhibitory effect on DNRA bacteria,the nrf A gene abundance showed as CK>CRU2>CRU1>NPK,and NPK treatment was significantly different from CK(P<0.05).The dominant phylum of DNRA bacteria in rice soil were Proteobacteria,Verrucomicrobia and Planctomycetes and so on.The relative abundance of Planctomycetes was increased under nitrogen fertilization,while the relative abundance of Proteobacteria was decreased.The relative abundance of Proteobacteria in CRU1 treatment was the lowest but that of Planctomycetes was the highest.RDA analysis showed that Proteobacteria was positively correlated with soil pH and total sulfur,negatively correlated with organic matter,total nitrogen,nitrate nitrogen and ammonium nitrogen,and Planctomycetes was positively correlated with organic matter,total nitrogen and hydrolyzable nitrogen,negatively correlated with soil pH,total sulfur and ferrous iron.The Shannon and Simpson diversity indices were the highest in CRU1 treatment.Therefore,the application of controlled-release nitrogen fertilizer improved the microbial community and structure of the DNRA process in paddy soil,which was beneficial to the nitrogen retention in the field ecosystem.关键词
长期不同施氮/控释氮肥/DNRA群落丰度与结构Key words
long-term different nitrogen application/controlled-release nitrogen fertilizer/DNRA community abundance and structure引用本文复制引用
张艺,孙梅,龙泽东,聂三安,田昌,黄晶,姚莉,孙耿..双季稻田土壤DNRA菌群落丰度与结构对不同施氮的响应[J].中国土壤与肥料,2025,(5):79-86,8.基金项目
湖南省教育厅重点项目(23A0185) （23A0185）
长沙市基金(kq2208129) （kq2208129）
湖南省农业创新资金项目(2024CX38). （2024CX38）
