天津中医药大学学报2025，Vol.44Issue(6)：519-526,8.DOI:10.11656/j.issn.1673-9043.2025.06.07
vlPAG脑区P2X3受体参与电针缓解IBS小鼠内脏高敏感的机制研究
Mechanistic study on P2X3 receptors in the ventrolateral periaqueductal gray underlying electroacupuncture-induced relief of visceral hypersensitivity in irritable bowel syndrome mice
摘要
Abstract
[Objective]To investigate the role of ventrolateral periaqueductal gray(vlPAG)in irritable bowel syndrome(IBS)mice with visceral hypersensitivity by in-vivo calcium fiber optic recording.To investigate the mechanism of P2X3 receptor in vlPAG participating in relieving visceral hypersensitivity in IBS mice with electroacupuncture by patch-clamp recording.[Methods]Male C57 and Vgat-Cre mice were subjected to enema with 2,4,6-trinitrobrnzen sulfonic acid(TNBS)to prepare IBS model with visceral hypersensitivity.Four weeks after model establishment,interventions including electroacupuncture(EA)and intracerebral cannula-guided drug administration were implemented.Successful establishment of the IBS visceral hypersensitivity model was confirmed by Hematoxylin-eosin(HE)staining and abdominal withdrawal reflex(AWR)score.The changes of visceral hypersensitivity in IBS mice was assessed using AWR score,the activity changes of vlPAG neurons in IBS mice were observed by in-vivo calcium fiber optic recording,and the changes of membrane electrical characteristics of vlPAG neurons in IBS mice were detected by patch-clamp recording.[Results]1)Compared with the model group,the AWR scores of mice in EA group were obviously decreased at all four levels of colorectal distention(CRD)stimulation(P＜0.01).2)Under 45 mmHg CRD stimulation,calcium flow in glutamergic(Glu)neurons in vlPAG of mice in EA group decreased and calcium flow in GABAergic(GABA)neurons increased compared with model group.3)Compared with model group,the AWR scores of mice in P2X3 receptor agonist group were decreased in all four levels of CRD stimulation(P＜0.05).4)Compared with model group,the number of action potentials of vlPAG neurons in EA group and P2X3 receptor agonist group were increased(P＜0.05).[Conclusion]EA can change the electrophysiological characteristics of neurons by regulating the expression of P2X3 receptor in vlPAG of IBS mice,which may be one of the central sensitization mechanisms of EA to relieve the visceral pain in IBS mice.关键词
肠易激综合征/电针/中腹外侧区脑导水管周围灰质/P2X3受体/内脏高敏感Key words
irritable bowel syndrome/electroacupuncture/ventrolateral periaqueductal gray/P2X3 receptor/visceral hypersensitivity分类
医药卫生引用本文复制引用
张方,王馨悦,翁志军,陈子怡,朱璐,马喆,杨玲,吴焕淦,刘慧荣..vlPAG脑区P2X3受体参与电针缓解IBS小鼠内脏高敏感的机制研究[J].天津中医药大学学报,2025,44(6):519-526,8.基金项目
国家自然科学基金青年项目(81904301,82105012) （81904301,82105012）
上海市自然科学基金项目(22ZR1458600) （22ZR1458600）
上海市人才发展资金资助项目(2021058) （2021058）
上海市"科技创新行动计划"启明星项目(扬帆专项)(23YF1442100). （扬帆专项）
