天津中医药大学学报2025，Vol.44Issue(6)：527-535,9.DOI:10.11656/j.issn.1673-9043.2025.06.08
Piezo2介导针刺得气客观反应及镇痛效应的机制研究
Study on the Mechanism of Piezo2-mediated Objective Deqi Response and Analgesic Effect of Acupuncture
摘要
Abstract
[Objective]To make the construction of a research platform for the objective Deqi response in acupuncture experimental animals and to conduct the exploration of whether the Piezo2 channel in the acupoint area mediates the objective Deqi response and analgesic effect of acupuncture.[Methods]In Experiment 1,the random division of SD rats into a model group,a non-manipulation group,and a manipulation group was conducted.The establishment of the model by the subcutaneous injection of 0.1 mL Complete Freund's Adjuvant(CFA)into the right plantar was conducted.In the manipulation group,the"Zusanli"(ST36)acupoint of the rat was inserted 3～5 mm deep,stimulated by rotating manipulation at 180 times/min for 2 minutes,with a 5-minute interval before the next needle manipulation,repeated 4 times,once daily for 3 days.The non-manipulation group was inserted to the same depth but retained for 30 minutes without manipulation.The measurement of the local electromyographic(EMG)response during acupuncture and the pulling force of the needle upon withdrawal as objective indicators of Deqi was conducted;the paw withdrawal latency(PWL)to thermal pain was measured fir the evaluation of the pain effect;and the activation of Piezo2-positive neurons in the L4 dorsal root ganglion(DRG)was detected by immunofluorescence staining.In Experiment 2,the random division of rats into model+control liquid group,model+Piezo2 inhibitor group,manipulation+control liquid group,and manipulation+Piezo2 inhibitor group was conducted,with 6 rats in each group.20 minutes before acupuncture,20 μL of control liquid(pure water)or inhibitor was injected into the muscle of the"Zusanli"acupoint area.The manipulation and measurement indicators were the same as in Experiment 1.[Results]In contrast with non-manipulation,the thermal radiation pain threshold of the right plantar in AIA model rats could be significantly increased by acupuncture manipulation,EMG discharges in the acupoint area could be induced,and the pulling force of the needle could be increased,with Piezo2-expressing neurons in the L4 DRG being significantly activated.When the Piezo2 channel inhibitor was injected into the acupoint area before acupuncture,the activation ratio of Piezo2-positive neurons in the L4 DRG was decreased,the EMG discharges in the acupoint area were weakened,the pulling force of the needle was reduced,and the analgesic effect of acupuncture was blocked.[Conclusion]The objective Deqi response can be induced and an analgesic effect can be produced in CFA rats through acupuncture manipulation.Piezo2 is mediated in the generation of the objective Deqi response and analgesic effect,and is one of the key pathways for the initiation of the acupuncture effect in the acupoint area.关键词
针刺/得气/镇痛/肌电/Piezo2Key words
acupuncture/Deqi/analgesia/EMG/Piezo2分类
医药卫生引用本文复制引用
翟永晓,刘雪,高卫芳,吴勇箭,蒙小雨,裴武博,刘阳阳,郭义..Piezo2介导针刺得气客观反应及镇痛效应的机制研究[J].天津中医药大学学报,2025,44(6):527-535,9.基金项目
国家自然科学基金重点项目(82030125). （82030125）
