信阳师范大学学报（自然科学版）2025，Vol.38Issue(3)：325-334,10.DOI:10.3969/j.issn.2097-583X.2025.03.011
异化硝酸盐还原为铵功能菌株的筛选及产铵能力的优化
Screening of functional strains of dissimilatory nitrate reduction to ammonium and optimization of ammonium production capacity
摘要
Abstract
The issue of nitrogen loss in paddy soils was addressed,where nitrate nitrogen was prone to dissimilatory reduction to gaseous nitrogen.The research focused on the targeted screening of microorganisms in paddy soils that can dissimilate nitrate to ammonium,thereby retaining nitrogen.The selected strains were identified using morphological and 16S rDNA gene sequencing techniques.Further,the ammonium production capability of these strains was optimized through single factor experiments.The results indicated that strain S2,identified as Enterobacter asburiae,exhibited a strong ability to dissimilate nitrate to ammonium.Optimization experiments revealed that the optimal carbon source for the growth of Enterobacter asburiae S2 was sodium citrate,the optimal pH for growth was 7.0,the optimal carbon-to-nitrogen ratio was found to be 25,under which the maximum ammonium production increased to 34.84 mg/L.关键词
水稻土壤/氮素保留/硝酸盐异化还原为铵/功能菌株/培养条件优化Key words
paddy soil/nitrogen retention/dissimilatory nitrate reduction to ammonium/functional strains/culture condition optimization分类
生物科学引用本文复制引用
史明子,张森,张警予,张斌,王赐卫,满振团,王明道,崔光周..异化硝酸盐还原为铵功能菌株的筛选及产铵能力的优化[J].信阳师范大学学报（自然科学版）,2025,38(3):325-334,10.基金项目
河南省自然科学青年基金项目(242300420606) （242300420606）
河南省烟草公司信阳市公司科技创新项目(信阳优质烟土壤微生态调控菌剂的开发与应用,30802153) （信阳优质烟土壤微生态调控菌剂的开发与应用,30802153）
