云南师范大学学报（自然科学版）2025，Vol.45Issue(3)：35-42,8.DOI:10.7699/j.ynnu.ns-2025-030
马铃薯抗青枯病材料筛选和表型鉴定
Screening and Phenotypic Identification of Materials Resistant to Bacterial Wilt in Potato
摘要
Abstract
Bacterial wilt caused by Ralstonia solanacearum is the most devastating bacterial disease af-fecting potato(Solanum tuberosum L.).This study unraveled the multi-layered synergistic defense mechanisms of resistant germplasm C804 against R.solanacearum strain P2 through physiological,bio-chemical,and spatiotemporal transcriptomic analyses.Results demonstrated that C804 achieves high-efficiency resistance via an enzyme-metabolism-gene three-in-one network.At the physiological level,its SOD and POD cascade precisely scavenges ROS,while proline metabolic reprogramming maintains oxidative homeostasis.Susceptible genotype A056 exhibits delayed enzymatic activity and aggravated membrane lipid peroxidation,leading to defense dysregulation.At the molecular level,C804 establishes spatiotemporal defense through the ScPR1-ScMLP1 dual-gene module:ScPR1 shows explosive upreg-ulation in roots(6.2-fold)and stems(8.5-fold),activating systemic acquired resistance,while ScM-LP1 specifically overexpresses at mid-infection stage(78.9-fold in roots,239.3-fold in stems)to rein-force physical barriers.关键词
马铃薯/青枯病/生理指标/转录组/抗病基因Key words
Potato/Bacterial wilt/Physiological parameters/Transcriptome/Resistance gene分类
农业科技引用本文复制引用
陈春,王艳梅,范满,林思婷,李世明,朱元涌,赵强彪,祝光涛,王娟..马铃薯抗青枯病材料筛选和表型鉴定[J].云南师范大学学报（自然科学版）,2025,45(3):35-42,8.基金项目
云南省科学技术厅基础研究计划资助项目(202501CF070007) （202501CF070007）
云南省教育厅基础研究基金资助项目(2025J0132). （2025J0132）
