中国美容医学2025，Vol.34Issue(7)：1-6,6.
牛蒡子苷元调节Hippo信号通路对黑素瘤细胞化疗耐药性的影响
Impact of Arctigenin on Chemoresistance of Melanoma Cells by Regulating Hippo Signal Pathway
摘要
Abstract
Objective To explore the impact of arctigenin(ARG)on chemoresistance of melanoma cells and its regulation mechanism on Hippo signaling pathway in this process.Methods The drug resistant melanoma cell line A375/VEM was obtained by continuously culturing A375 cells with different concentrations of vemurafenib(VEM).CCK-8 was applied to detect the proliferative activity of VEM at different concentrations on A375 and A375/VEM cells,and the toxicity of ARG at different concentrations on A375/VEM cells.A375/VEM cells were grouped into control group,VEM group,ARG group,ARG+VEM group,and ARG+VEM+Hippo inhibitor group,CCK-8 method was applied to detect the survival rate of A375/VEM cells in each group,clone formation experiment was applied to detect the proliferation of A375/VEM cells in each group,flow cytometry was applied to detect the apoptosis of A375/VEM cells in each group.Western blot was applied to detect the expression of Cyclin D1,ERK1/2,p-ERK1/2,AKT,p-AKT,and Hippo pathway related proteins of cells in each group,the transplantation model of drug-resistant melanoma in mice was established,and the tumor growth was observed.Results Compared with the control group,the survival rate of A375/VEM cells,clone formation quantity,the expression of Cyclin D1,p-ERK1/2,p-AKT,and Yes-associated protein in the VEM group,ARG group,and ARG+VEM group reduced(P＜0.05),and the ARG+VEM group were lower than the VEM group and ARG group(P＜0.05),the apoptosis rate,and the levels of LATS1 and p-YAP increased(P＜0.05),and the ARG+VEM group were higher than the VEM group and ARG group(P＜0.05).Compared with the ARG+VEM group,the survival rate of A375/VEM cells,clone formation quantity,the expression of Cyclin D1,p-ERK1/2,p-AKT,and YAP in the ARG+VEM+Hippo inhibitor group increased(P＜0.05),the apoptosis rate,and the levels of LATS1 and p-YAP decreased(P＜0.05).In vivo experiments on nude mice transplanted with tumor cells showed that the tumor weight and volume in the ARG+VEM group were obviously lower than those in the VEM and ARG groups(P＜0.05).Further addition of Hippo pathway inhibitors revealed that the inhibitory effect of ARG on drug resistance in A375/VEM cells was reversed(P＜0.05).Conclusion ARG may inhibit the chemoresistance of melanoma cells by activating Hippo pathway.关键词
牛蒡子苷元(ARG)/黑素瘤细胞/化疗/耐药性/Hippo信号通路Key words
arctigenin(ARG)/melanoma cells/chemotherapy/resistance/hippo signaling pathway分类
医药卫生引用本文复制引用
高阳,杜紫微,王海飞..牛蒡子苷元调节Hippo信号通路对黑素瘤细胞化疗耐药性的影响[J].中国美容医学,2025,34(7):1-6,6.基金项目
河北省中医药管理局科研计划项目(编号:2021115) （编号:2021115）
