中国实验动物学报2025，Vol.33Issue(6)：779-789,11.DOI:10.3969/j.issn.1005-4847.2025.06.001
月桂酸钠诱导脑小血管病大鼠模型的建立与评价
Establishment and evaluation of a rat model of cerebral small vessel disease induced by sodium laurate
摘要
Abstract
Objective A rat model of cerebral small vessel disease(CSVD)was established by unilateral injection of a single dose of sodium laurate into the internal carotid artery.The effectiveness of the model was assessed by behavior scoring and analysis of serum-related indicators,cerebral infarction volume,cerebral microvascular density,hemodynamics,brain histopathology and the expression of blood-brain barrier(BBB)-related proteins.Methods SPF-grade male SD rats were divided randomly into a control group and a model group(n=6 per group).The model group received a single injection of 100 μL of sodium laurate(2 g/L)via the internal carotid artery,while the control group underwent the same surgical procedure but received an equal volume of saline.Neurobehavioral assessments were conducted using the Longa score and postural reflex test.Serum homocysteine(HCY)levels were measured by enzyme-linked immunosorbent assay.Cerebral infarction volume was detected by magnetic resonance imaging and changes in cerebral vascular density were observed by cerebrovascular imaging.The resistance index(RI)and perfusion index(PI)were measured by ultrasonography.Histopathological changes in brain tissue were evaluated by hematoxylin and eosin(HE)staining.Expression of the cerebral microvascular marker CD31 and tight junction proteins ZO-1 and Occludin in brain cortex tissue were detected by immunohistochemical staining.Results The Longa score,postural reflex score(P＜0.05),and cerebral infarction volume were significantly increased(P＜0.05)while the cerebral vascular density was decreased in the model group compared with the control group.Serum HCY levels,carotid RI,and PI values were all significantly increased in the model group(P＜0.05).HE staining revealed solidified neuronal nuclei and enlarged perivascular spaces in the brain cortex in the model group.Immunohistochemical staining revealed that CD31,ZO-1,and Occludin expression were significantly reduced in the brain cortex in the model group compared with the control group(P＜0.05).Conclusions A rat model of CSVD can be established rapidly and effectively by a single unilateral injection of high-concentration sodium laurate via the internal carotid artery.This model is characterized by neurobehavioral abnormalities,cerebral infarction,insufficient cerebral blood supply,reduced vascular density,and disruption of the BBB,suggesting that it may serve as an effective rat model for the study of CSVD.关键词
脑小血管病/动物模型/血脑屏障/同型半胱氨酸/CD31Key words
cerebral small vessel disease/animal model/blood-brain barrier/homocysteine/CD31分类
生物科学引用本文复制引用
陈炎森,林浩伟,张予菲,林雨星,曹长源,赖可欣,吴钰婷,蔡巧燕,张铃..月桂酸钠诱导脑小血管病大鼠模型的建立与评价[J].中国实验动物学报,2025,33(6):779-789,11.基金项目
国家自然科学基金(82374282),福建省自然科学基金(2022J01876,2022J01875),国家级大学生创新创业训练计划项目(202310393006),福建中医药大学青年科技创新培育计划项目(XQC2024004).Funded by the National Natural Science Foundation of China(82374282),Natural Science Foundation of Fujian Province(2022J01876、2022J01875),National Innovation and Entrepreneurship Training Programme Project for College Students(202310393006),Fujian University of Traditional Chinese Medicine Youth Science and Technology Innovation Cultivation Program(XQC2024004). （82374282）
