中国实验动物学报2025，Vol.33Issue(6)：836-847,12.DOI:10.3969/j.issn.1005-4847.2025.06.006
槟榔碱对慢性不可预知温和应激小鼠抗抑郁作用及机制研究
Anti-depressant effect and mechanism of arecoline in mice with chronic and unpredictable mild stress
摘要
Abstract
Objective We explored the anti-depressant activity and mechanism of arecoline in vivo in a mouse model of depression induced by chronic unpredictable mild stress.The aim was to explore the possible mechanisms of action,providing experimental evidence for further research into the health benefits of arecoline and theoretical support for the scientific development and utilization of this resource.Methods Sixty quarantine-qualified SPF C57BL/6J mice were divided randomly into a control group,model group,fluoxetine group(20 mg/kg),and arecoline low-,medium-,and high-dose groups(10,20,and 40 mg/kg,respectively)according to body mass(n=10 mice per group).The effects of arecoline on the behavior of the mice were evaluated by open-field,tail-suspension,and forced-swimming tests.Serum corticosterone and serum and brain levels of superoxide dismutase(SOD)were detected by enzyme-linked immunoassay.Malondialdehyde(MDA),catalase(CAT),5-hydroxytryptamine(5-HT),and norepinephrine(NE)levels in brain tissue,and dopamine(DA),gamma-aminobutyric acid(GABA),tumor necrosis factor(TNF-α),interleukin(IL)-10,IL-1β,brain-derived neurotrophic factor(BDNF),tropomyosin receptor kinase B(TrkB),and cAMP-response element binding protein(CREB)were detected by Western Blot.Results Arecoline significantly reduced the total distance and average speed of the model mice in open field tests and increased activities,and significantly reduced the immobility time in the tail suspension and forced swimming tests.Arecoline also significantly decreased serum corticosterone levels,increased SOD and CAT,and decreased MDA levels.5-HT,DA,NE,and GABA levels were significantly increased,and the cytokines TNF-α,IL-6,and IL-1β were significantly decreased.Expression levels of BDNF,TrkB,and CREB in the brain tissue were significantly increased.Conclusions Research has found that arecoline has a significant antidepressant ability,and its mechanism may be achieved by reducing oxidative stress damage,inhibiting neuroinflammation,regulating neurotransmitter balance,and regulating the BDNF/TrkB/CREB signaling pathway..This study explored the antidepressant efficacy of arecoline and preliminarily revealed its possible regulatory mechanism,which can provide data support for the neuroactivity of arecoline and lay a theoretical foundation for the development of arecoline as medicine.关键词
槟榔碱/抗抑郁/氧化应激/神经炎症/神经递质Key words
arecoline/anti-depressant/oxidative stress/neuroinflammation/neurotransmitters分类
生物科学引用本文复制引用
王丹阳,崔竞雯,刘新民,范蓓,王凤忠,卢聪..槟榔碱对慢性不可预知温和应激小鼠抗抑郁作用及机制研究[J].中国实验动物学报,2025,33(6):836-847,12.基金项目
三亚中国农业科学院国家南繁研究院"南繁专项"(YBXM2417),三亚中国农业科学院国家南繁研究院"南繁专项"2022年院院联合攻关项目(YYLH05),中国农业科学院农产品加工研究所创新工程(CAAS-ASTIP-2024-IFST).Funded by Sanya China Academy of Agricultural Sciences National Research Institute of Southern Breeding"Southern breeding special Project"(YBXM2417),Sanya China Academy of Agricultural Sciences National Research Institute of Southern Breeding"Southern breeding special Project"2022 Joint Research Project(YYLH05),Innovation Project of Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2024-IFST). （YBXM2417）
