中国比较医学杂志2025，Vol.35Issue(6)：41-49,9.DOI:10.3969/j.issn.1671-7856.2025.06.005
基于miR-146a调控TLR4/NF-κB信号通路探讨加味四妙散对尿酸钠晶体诱导的RAW264.7细胞炎症的作用机制
Mechanism of Jiawei Simiao powder on monosodium urate crystal-induced RAW264.7 cell inflammation via miR-146a regulation of the Toll-like receptor 4/nuclear factor-κB signaling pathway
摘要
Abstract
Objective To investigate the effect of Jiawei Simiao powder on the Toll-like receptor 4(TLR4)/nuclear factor-κB(NF-κB)signaling pathway through miR-146a in monosodium urate(MSU)crystal-induced RAW264.7 macrophage inflammation models,and to explore its anti-inflammatory mechanism.Methods A cell model of gouty arthritis was established by inducing RAW264.7 cells with MSU crystals.Cells were divided into control,model,Jiawei Simiao powder,and colchicine groups and cell viability was assessed using the CCK-8 method.Levels of interleukin(IL)-1β,IL-6,and tumor necrosis factor(TNF)-α were measured by enzyme-linked immunosorbent assay.Expression levels of miR-146a miRNA and TLR4,myeloid differentiation factor 88(MyD88),TNF receptor-associated factor 6(TRAF6),and NF-κB p65 mRNA were detected by quantitative real-time polymerase chain reaction amplification technology,and protein expression levels of TLR4,MyD88,TRAF6,and phospho(p)-NF-κB p65 were evaluated by Western blot.Results miR-146a expression was significantly decreased in the model group compared with the control group(P＜0.01),while mRNA and protein expression levels of TLR4,MyD88,and TRAF6,and protein expression of p-NF-κB p65,IL-1β,IL-6,and TNF-α were significantly increased(P＜0.01).In contrast,miR-146a expression was significantly increased(P＜0.01)and mRNA and protein expression of TLR4,MyD88,and TRAF6,and p-NF-κB p65 protein expression(P＜0.01),as well as IL-1β,IL-6,and TNF-α levels(P＜0.05)were significantly decreased in the Jiawei Simiao powder and colchicine groups.Conclusions Jiawei Simiao powder may alleviate MSU crystal-induced macrophage inflammatory responses by upregulating miR-146a,suppressing TLR4/NF-κB signaling pathway activation,and decreasing secretion of inflammatory factors.关键词
加味四妙散/痛风性关节炎/miR-146a/TLR4/NF-κB信号通路Key words
Jiawei Simiao powder/gouty arthritis/miR-146a/TLR4/NF-κB signaling pathway分类
医药卫生引用本文复制引用
李华燕,苏培培,王鑫,牛源源,陈禛恒,孙千惠,杜明瑞..基于miR-146a调控TLR4/NF-κB信号通路探讨加味四妙散对尿酸钠晶体诱导的RAW264.7细胞炎症的作用机制[J].中国比较医学杂志,2025,35(6):41-49,9.基金项目
河南省中医药传承与创新人才工程(仲景工程)中医药学科拔尖人才项目(CZ0325-15) （仲景工程）
河南省卫生健康委员会国家中医药传承创新中心联合共建科研专项(2024ZXZX1052). （2024ZXZX1052）
