中国比较医学杂志2025，Vol.35Issue(6)：65-73,9.DOI:10.3969/j.issn.1671-7856.2025.06.007
基于Nrf2/ARE通路探究五味子多糖对运动性疲劳小鼠骨骼肌线粒体自噬的影响
Effect of Schisandra chinensis polysaccharide on mitochondrial autophagy in skeletal muscle in exercise-fatigue mice based on the nuclear factor erythroid 2-related factor 2/antioxidant response element pathway
摘要
Abstract
Objective To investigate the effect of Schisandra chinensis polysaccharide(SCP)on mitochondrial autophagy in skeletal muscle in exercise-fatigue mice based on the nuclear factor erythroid 2-related factor 2(Nrf2)/antioxidant response element ARE pathway.Methods A fatigue mouse model was established by swimming training.Mice were assigned randomly to fatigue,SCP(20 mg/kg),Rhodiola rosea(8 mL/kg),inhibitor(30 mg/kg ML385),and SCP+inhibitor groups,with normal mice as the control group.Serum was separated and levels of lactic acid(LA),lactate dehydrogenase(LDH),blood urea nitrogen(BUN),and creatine kinase(CK)were detected.Liver tissue was also isolated and levels of malondialdehyde(MDA),hepatic glycogen(HG),and superoxide dismutase(SOD)were detected.Skeletal muscle tissues were isolated and muscle glycogen(MG),changes in skeletal muscle tissue in exercise fatigue,and mitochondrial morphology were detected.Gene expression levels of mitochondrial transcription factor A(TFAM)and nuclear respiratory factor-1(NRF-1),and Nrf2,quinone oxidoreductase 1(NQO1),heme oxygenase-1(HO-1),and LC3 protein expression levels were analyzed.Results HG,MG,SOD,TFAM mRNA,NRF-1 mRNA,Nrf2,NQO1,HO-1,and LC3 Ⅱ/LC3 Ⅰ were lower in the fatigue group than in the control group,while levels of LA,BUN,LDH,CK,and MDA were higher than in the control group(P＜0.05).HG,MG,SOD,TFAM mRNA,NRF-1 mRNA,Nrf2,NQO1,HO-1,and LC3 Ⅱ/LC3 Ⅰ levels were higher in the SCP and Rhodiola rosea groups than in the fatigue group,while LA,BUN,LDH,CK,and MDA levels were lower than in the fatigue group(P＜0.05).The above indexes were opposite in the inhibitor group(P＜0.05).Conclusions SCP promotes mitochondrial autophagy in skeletal muscle in exercise-fatigue mice by activating the Nrf2/ARE pathway,effectively exerting anti-fatigue effects.关键词
五味子多糖/运动性疲劳/线粒体自噬/骨骼肌/Nrf2/ARE通路Key words
Schisandra chinensis polysaccharide/exercise fatigue/mitochondrial autophagy/skeletal muscle/Nrf2/ARE pathway分类
医药卫生引用本文复制引用
蒋军文,覃丽雯,沈仰峰..基于Nrf2/ARE通路探究五味子多糖对运动性疲劳小鼠骨骼肌线粒体自噬的影响[J].中国比较医学杂志,2025,35(6):65-73,9.基金项目
广西高校中青年教师科研基础能力提升项目(2024KY1781) （2024KY1781）
广西高等教育本科教学改革工程项目(2024JGA459). （2024JGA459）
