浙江医学2025，Vol.47Issue(12)：1263-1271,9.DOI:10.12056/j.issn.1006-2785.2025.47.12.2025-279
hsa_circ_0038817在头颈部鳞状细胞癌中的表达及作用机制研究
Expression and functional mechanism of hsa_circ_0038817 in head and neck squamous cell carcinoma
摘要
Abstract
Objective To explore the expression of circular RNA(circRNA)hsa_circ_0038817 in head and neck squamous cell carcinoma(HNSCC)and its potential regulatory mechanisms.Methods RNA sequencing was employed to illustrate the expression profile of circRNAs in HNSCC.Subsequently,bioinformatics analysis,sequencing by Sanger dideoxychain termination method,and quantitative real-time PCR were utilized to ascertain the ring-forming traits of RNA and dissect its expression patterns.The influence of hsa_circ_0038817 on cell growth and proliferation was appraised through colony formation assays,wound healing assays,and Transwell assays.Additionally,microRNA target gene database screening,luciferase reporter gene assays,and Western blotting were used to probe into the underlying mechanism of hsa_circ_0038817 regulating HNSCC invasion.Results In the context of HNSCC,hsa_circ_0038817 exhibited an obviously elevated expression level both in cancer tissues and cells,and its loop structure was verified.The overall survival was low in patients with high hsa_circRNA_0038817 expression level with the expression level closely related to the clinical stage,and lymph-node metastasis and living status.Meanwhile,in vitro experiments validated that the knockdown of hsa_circ_0038817 led to the suppression of HNSCC cell migratory and invasive capabilities,as well as the epithelial-to-mesenchymal transition process.From the perspective of mechanism,hsa_circ_0038817 down-regulated the expression of high mobility group AT-hook 2(HMGA2)byacting as a molecular sponge of miR-203,thereby facilitating the progression of HNSCC.Conclusion hsa_circ_0038817 is highly expressed in HNSCC tissues,and interacts with the miR-203/HMGA2 axis through a competitive endogenous RNA regulatory mechanism,promoting HNSCC cell proliferation.These findings suggest that hsa_circ_0038817 may serve as both a prognostic biomarker and a potential therapeutic target for HNSCC.关键词
环状RNA/头颈部鳞状细胞癌/微小RNA/高迁移率族蛋白A2Key words
Circular RNA/Head and neck squamous cell carcinoma/MicroRNA/High mobility group AT-hook protein 2引用本文复制引用
李群,许雨欣,史晨雪,邬振华,王剑,沈志森..hsa_circ_0038817在头颈部鳞状细胞癌中的表达及作用机制研究[J].浙江医学,2025,47(12):1263-1271,9.基金项目
浙江省医药卫生科技计划项目(2024KY282) （2024KY282）
宁波市医疗卫生高端团队重大攻坚项目(2023030514) （2023030514）
宁波市临床医学研究中心项目(2022L005) （2022L005）
宁波市医疗中心李惠利医院"惠利基金"(2022ZD001) （2022ZD001）
