浙江医学2025，Vol.47Issue(12)：1272-1276,1283,后插5-后插6,8.DOI:10.12056/j.issn.1006-2785.2025.47.12.2024-2161
幽门螺杆菌感染对胃溃疡患者胃黏膜微生物群结构及生物学功能的影响
Impact of Helicobacter pylori infection on the structure and biological functions of the gastric mucosal microbiota in patients with gastric ulcer
摘要
Abstract
Objective To explore the impact of Helicobacter pylori(Hp)infection on the structure and biological functions of gastric mucosal microbiota in patients with gastric ulcer from a metagenomic perspective.Methods Eight patients with gastric ulcers who underwent gastroscopy at the First People's Hospital of Xiaoshan District,Hangzhou from March to June,2024 were selected as the observation group.In addition,eight healthy individuals aged 30-55 years with normal gastroscopy results at the same hospital during the same period were selected as the control group.Metagenomic sequencing was used to analyze the composition of the microbial community and the metabolic functions of related genes in the gastric mucosa tissue of the two groups of research subjects.Results In the observation group with Hp-positive patients,the gastric mucosal microbiota was dominated by Hp,and the biodiversity was significantly lower than that of the control group(P＜0.05).In addition,compared to the control group,13 differential functional pathways were identified in the observation group,mainly involving energy metabolism,nucleotide and nucleotide biosynthesis,vitamin and cofactor biosynthesis,nucleotide metabolism,and other biosynthetic pathways.The differential functions of mucosal microorganisms were all derived from Hp,including queuosine-transfer RNA modification pathways and pathways related to demethylna-phthoquinone/naphthoquinone synthesis.Conclusion Hp infection significantly alters the structure,diversity,and biological functions of the gastric microbiota,potentially leading to mucosal damage and ulcer formation.关键词
幽门螺杆菌/胃溃疡/宏基因组测序/Q-tRNA修饰/甲萘醌Key words
Helicobacter pylori/Gastric ulcer/Metagenomic sequencing/Queuosine-modified tRNA/Menaquinone引用本文复制引用
宣涵,梁军才,郭锋,王敏,金婷,张海翔,张磊..幽门螺杆菌感染对胃溃疡患者胃黏膜微生物群结构及生物学功能的影响[J].浙江医学,2025,47(12):1272-1276,1283,后插5-后插6,8.基金项目
杭州市医药卫生科技项目(B20220680、B20220939) （B20220680、B20220939）
