浙江医学2025，Vol.47Issue(12)：1277-1283,后插6,8.DOI:10.12056/j.issn.1006-2785.2025.47.12.2025-117
补虚祛湿消癥方对IgA肾病大鼠的治疗效果及其作用机制研究
Efficacy and mechanism of Buxu Qushi Xiaozheng Formula on rats with IgA nephropathy
摘要
Abstract
Objective To explore the efficacy and mechanism of the Buxu Qushi Xiaozheng Formula(BXQSXZ)on a rat model of immunoglobulin A nephropathy(IgAN).Methods Twenty-five male SD rats were divided into negative control group,model group,positive control group(Benazepril),low-dose BXQSXZ group,and high-dose BXQSXZ group,5 rats for each.The IgAN rat model was established using oral immunogen,subcutaneous injection of carbon tetrachloride,and intravenous injection of lipopolysaccharide.After 4 weeks of gavage administration,24-hour urinary total protein(UTP),blood urea nitrogen(BUN),creatinine(Cr),IgA1-related immune factors in each group were measured.Kidney tissue was isolated,and immunofluorescence staining was used to detect IgA content in the kidney.Hematoxylin and eosin staining was performed to observe pathological changes in kidney tissue.After homogenizing kidney tissue,the levels of inflammatory factors in kidney tissue were measured.Expression levels of core 1 β1,3-galactosyltransferase(C1GALT1)and its chaperone protein(COSMC)were detected by real-time polymerase chain reaction and Western blot.Results Compared to the normal control group,a large amount of IgA deposition was observed in the glomerular mesangial area of the model rats.Compared to the IgAN model group,the high-dose BXQSXZ group showed reduced renal IgA content in kidney tissue,decreased serum levels of IgA1,galactose-deficient IgA1,B-cell-activating factor,and proliferation-inducing ligand(all P＜0.05),and lowered UTP,Cr,and BUN levels(all P＜0.05),as well as improved glomerular and interstitial lesions,reduced levels of Toll-like receptor 4,tumor necrosis factor-α,interleukin-6(IL-6),and IL-4 in kidney tissues(all P＜0.05),and elevated IL-10 and interferon-γ levels(all P＜0.05).Additionally,C1GALT1 and COSMC mRNA and protein expression in kidney tissues were all elevated(all P＜0.05).Conclusion BXQSXZ can inhibit the production and deposition of IgA-related immune complexes in the kidney tissue of IgAN rats,improve urinary protein,renal function,and kidney tissue lesions,and alleviate renal inflammatory responses,possibly by restoring the expressions of C1GALT1 and COSMC.关键词
免疫球蛋白A肾病/补虚祛湿消癥方/肾功能不全/核心1β1,3-半乳糖基转移酶Key words
Immunoglobulin A nephropathy/Buxu Qushi Xiaozheng Formula/Renal insufficiency/Core 1 β1,3-galactosyltransferase引用本文复制引用
邢洁,高骏伟,谢文佳,蔡旭东..补虚祛湿消癥方对IgA肾病大鼠的治疗效果及其作用机制研究[J].浙江医学,2025,47(12):1277-1283,后插6,8.基金项目
浙江省中医药科技计划项目(2023ZL152) （2023ZL152）
浙江省中医药重点学科建设项目(2024-XK-63) （2024-XK-63）
宁波市中医肾病临床医学研究中心重大攻关项目(2024L001) （2024L001）
