中医药学报2025，Vol.53Issue(7)：116-124,后插1,10.DOI:10.19664/j.cnki.1002-2392.250154
重楼综合形态学、显微学真伪鉴别研究进展及ITS2序列分析研究
Research Progress on Comprehensive Morphological and Microscopic Authentication of Paris polyphylla and ITS2 Sequence Analysis
摘要
Abstract
Based on identification techniques including morphological characteristics,microscopic features,physicochemical properties and DNA sequences,this paper reviews and analyzes the differences between genuine Paris polyphylla and its adulterants to provide references for authentication.The varieties of genuine P.polyphylla and its adulterants were summarized based on literature,including differences in original plant morphology and microscopic characteristics.The ITS2 sequences of P.polyphylla and its adulterants were downloaded from public databases,standardized based on Hidden Markov Model principles,and subjected to sequence alignment,secondary structure prediction and phylogenetic tree cluster analysis.The main adulterants of genuine P.polyphylla include P.fargesii,P.latifolia,P.thibetica and P.forrestii,while common counterfeits are Polygonum bistorta,Polygonum viviparum and Typhonium giganteum.Significant differences exist between genuine and adulterant materials in plant morphology(stem,leaf,flower,fruit and seed)and microscopic identification,while crude drug characteristics and microstructures show minor differences.In DNA sequence identification,the ITS2 sequences show minor differences between genuine and adulterant materials but significant differences from counterfeits.Genuine and adulterant materials can be effectively distinguished by plant morphology,while ITS2 sequences and their secondary structures can rapidly and accurately identify counterfeits when only crude drugs are available.This study provides references for clinical safety and quality evaluation of P.polyphylla,and may also serve as reference for authentication of other medicinal materials.关键词
重楼/混伪品/形态鉴别/性状鉴别/显微鉴别/ITS2 序列Key words
Paris polyphylla/Adulterants/Morphological identification/Character identification/Microscopic identification/ITS2 sequence分类
医药卫生引用本文复制引用
杨志城,汲虹,孙彩虹,叶亮,叶冠..重楼综合形态学、显微学真伪鉴别研究进展及ITS2序列分析研究[J].中医药学报,2025,53(7):116-124,后插1,10.基金项目
上海医药中药传承和创新平台能力建设(2020006) （2020006）
