中国现代医生2025，Vol.63Issue(18)：9-13,17,6.DOI:10.3969/j.issn.1673-9701.2025.18.003
儿童暴发性心肌炎miRNA的筛选及生物信息学分析
Screening and bioinformatics analysis of expressed miRNA in pediatric fulminant myocarditis
摘要
Abstract
Objective To analyze the differentially expressed microRNA(miRNA)and their target genes in the serum of pediatric patients with fulminant myocarditis(FM)through bioinformatics methods,and to explore the pathogenesis.Methods GSE221090 dataset from the high-throughput Gene Expression Omnibus(GEO)were selected,and bioinformatics analysis was performed by using the GEO2R online tool to screen for differentially expressed miRNA.The online miRDB database was used to predict the target genes of the differentially expressed miRNA.The DAVID tool was employed for gene ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis of the screened target genes.Additionally,protein-protein interaction networks(PPI)associated with the differentially expressed genes was constructed by using STRING database and Cytoscape software,and core genes were screened.Results A total of 148 differentially expressed miRNA were identified in the serum of pediatric FM group compared to normal children group,including 109 up-regulated and 39 down-regulated miRNA.The top ten up-regulated and down-regulated miRNA based on their scores were selected,and target gene prediction for a forementioned miRNA was conducted by using the online miRDB database,identifying 291 target genes regulated by up-regulted miRNA and 290 target genes regulated by down-regulated miRNA.Subsequent GO and KEGG analyses demonstrated that the target genes of up-regulated miRNA were primarily enriched in signaling pathways including phosphatidylinositol 3-kinase/protein kinase B signaling pathway(PI3K/Akt)and forhead box O,whereas the target genes of down-regulated miRNA predominantly participated in the transforming growth factor-β(TGF-β)signaling pathway and related pathways.The top ten differentially expressed genes with the highest relevance scores using PPI and Cytoscape software were identified,including sirtuin 1(SIRT1),signal transducer and activator of transcription 3,estrogen receptor 1,H3.3 histone B,nuclear receptor corepressor 1,interferon regulatory factor 4,interleukin-1 Beta,dicer 1 ribonuclease Ⅲ,histone deacetylase 1(HDAC1),and DEAD-box helicase.Conclusion miR-22-3p,miR-4284,and others are crucial in the pathogenesis of pediatric FM,possibly related to the expression levels of SIRT1 and HDAC1 regulated,with mechanisms that may exert biological effects via the PI3K/Akt,TGF-β,and other signaling pathways.关键词
暴发性心肌炎/微小RNA/基因芯片/生物信息学Key words
Fulminant myocarditis/microRNA/Gene chip/Bioinformatics分类
医药卫生引用本文复制引用
王陆银,姚家芳,唐坎凯..儿童暴发性心肌炎miRNA的筛选及生物信息学分析[J].中国现代医生,2025,63(18):9-13,17,6.基金项目
浙江省湖州市科学技术局项目(2024GYB24) （2024GYB24）
