浙江农业学报2025，Vol.37Issue(5)：977-986,10.DOI:10.3969/j.issn.1004-1524.20240204
玉米生物钟基因ZmPRR1-2互作蛋白质的筛选
Screening of proteins interacting with circadian clock gene ZmPRR1-2 in maize
摘要
Abstract
The pseudo-response regulatory protein TOC1,one of the five members of the pseudo-response regulator(PRR)family proteins,as the core component of the central oscillator of clock,together with the MYB-like tran-scription factors LHY and CCA1,constitute the central negative regulatory feedback loop of the central oscillator of clock,which plays an important role in the circadian clock system.The ZmPRR1-2 gene is a homologous gene of TOC1 in Arabidopsis.In order to deeply explore the biological function of ZmPRR1-2,the proteins interacting with ZmPRR1-2 was screened from the yeast cDNA library of a tropical maize inbred line induced by long-day photoperiod treatment by yeast two-hybrid technique.The results showed that the bait vector PGBKT7-ZmPRR1-2 was not toxic to yeast strain and had no self-activation effect on the reporter gene.A total of 12 proteins containing the full length in-teracting with ZmPRR1-2 were identified.These candidate interacting proteins were involved in many pathways,such as response to oxidative stress,response to oxidative stress,response to aluminum ion,photosynthesis,electron transport chain,light harvesting,auxin-activated signaling pathway,regulation of DNA-templated transcription,tryp-tophan biosynthetic process,translation,immune response,positive regulation of apoptotic signaling pathway and proteolysis involved in protein catabolic process.It is speculated that ZmPRR1-2 protein may participate in multiple signal transduction and metabolic pathways by interacting with the identified interaction proteins.The results might improve our understanding of the further study of the molecular function and regulatory mechanism of ZmPRR1-2.关键词
玉米/生物钟/ZmPRR1-2/酵母双杂交/互作蛋白质Key words
maize/circadian/ZmPRR1-2/yeast two-hybrid system/interacting protein分类
农业科技引用本文复制引用
王闻琦,王盼盼,张严玲,刘青青,洪双双,赵高鹏,刘泓畅,王翠玲..玉米生物钟基因ZmPRR1-2互作蛋白质的筛选[J].浙江农业学报,2025,37(5):977-986,10.基金项目
国家自然科学基金河南省联合基金(U2004153) （U2004153）
