浙江农业学报2025，Vol.37Issue(5)：1029-1044,16.DOI:10.3969/j.issn.1004-1524.20240463
基于串联质量标签(TMT)技术的宁都黄鸡大小睾丸蛋白质组学研究
Proteomic study of large and small testes in Ningdu Yellow chicken based on tandem mass tags(TMT)technology
摘要
Abstract
This experiment aimed to investigate the protein expression profiles in testes of adult Ningdu Huang chick-ens,screen differentially expressed proteins and functional pathways between individuals with large and small testes,and provide foundational data for breeding selection of reproductive performance.Using testicular tissues from six 22-week-old Ningdu Huang chickens divided into large-testis(H-TES)group and small-testis(L-TES)group,tandem mass tags(TMT)technology was employed to conduct differential expression analysis,functional enrichment analy-sis,and gene interaction analysis of testicular proteins between the two groups.Candidate proteins were preliminarily screened,and correlations between the expression levels of these proteins in six individuals and six testicular trait in-dicators(left testis weight,right testis weight,total testis weight,left testis index,right testis index,total testis in-dex)were analyzed to investigate protein functions.Results showed that 4 683 proteins were identified in Ningdu Huang chicken testes,with 144 differentially expressed proteins screened.GO analysis revealed that these differenti-al expressed proteins primarily participate in sperm structural composition and possess functions including ubulin-glu-tamic acid ligase activity,thymine binding,and dehydroascorbic acid transmembrane transporter activity,etc.Sig-nificantly enriched KEGG pathways included arginine and proline metabolism,pantothenate and CoA biosynthesis,and β-alanine metabolism,etc.The protein interaction network contained 34 differential proteins,and 30 candidate proteins were preliminarily identified.Through correlation analysis and literature review,16 key candidate proteins were screened:SPAG6,TPPP2,ENKUR,ODF2,SAXO1,TCP11,DNALI1,CCDC63,TSSK3,TEKT2,TEKT3,ALDH2,DNAH5,CCDC40,CCDC39,and DNAH10.Based on their functions,key regulatory KEGG pathways for testicular traits were identified as arginine and proline metabolism,pantothenate and CoA biosynthesis,β-alanine metabolism,tight junction,and gap junction.These findings provide fundamental data for breeding selection of tes-ticular quality traits in local chicken breeds.关键词
宁都黄鸡/睾丸/TMT/蛋白质组学/候选基因Key words
Ningdu yellow chicken/testis/tandem mass tag(TMT)/proteome/candidate gene分类
农业科技引用本文复制引用
马荆鄂,吴斯琪,万淑敏,熊信威,许继国,许晶,饶友生,周敏..基于串联质量标签(TMT)技术的宁都黄鸡大小睾丸蛋白质组学研究[J].浙江农业学报,2025,37(5):1029-1044,16.基金项目
国家自然科学基金(32160784) （32160784）
江西省高等学校教学改革研究重点课题(JXJG-23-23-5) （JXJG-23-23-5）
