浙江农业学报2025，Vol.37Issue(5)：1045-1056,12.DOI:10.3969/j.issn.1004-1524.20240886
弧菌噬菌体鸡尾酒制剂对凡纳滨对虾肠道微生物区系的影响
Effect of Vibrio phage cocktail on the intestinal microbiota of Litopenaeus vannamei
摘要
Abstract
This experiment aimed to investigate the effects of a phage cocktail preparation on the intestinal microbiota of Litopenaeus vannamei.Vibrio strains were isolated and identified from canvas-pond aquaculture environments,while the antibacterial efficacy of the phage cocktail against Vibrio was evaluated.Two experimental groups were es-tablished,each with three replicate culture tanks.The phage group was fed with a diet containing the phage cocktail preparation,while the control group was fed with a standard diet.Differences in intestinal microbiota were analyzed using 16S rRNA high-throughput sequencing.Three Vibrio strains were isolated from the canvas ponds:DXA(Vibrio owensii),DXD(V.harveyi),and DXF(Vibrio sp.VibC-Oc-076),with the phage cocktail demonstrating effective inhibition against all three strains.Shrimp fed the phage cocktail exhibited significantly increased intestinal microbial richness and more stable bacterial community structures.At the phylum level,Bacteroidota,Proteobacteria,and Verrucomicrobiota dominated both groups,with no significant change observed in Bacteroidota abundance in the phage group.At the genus level,Algoriphagus and Ruegeria abundances initially increased then decreased in the phage group,while Muricauda abundance showed an opposite trend.The Vibrio genus abundance in shrimp intestines remained below 0.3％.Although Vibrio counts decreased on the second day of feeding with the phage cocktail,no significant difference was observed compared to the control group.In conclusion,the Vibrio-targeting phage cocktail enhanced the diversity of intestinal microbiota in L.vannamei and stabilized the gut microecological environment.However,its impact on Vibrio bacteria appeared related to baseline abundance levels,warranting further research to elucidate this correlation.关键词
凡纳滨对虾/弧菌/噬菌体鸡尾酒制剂/微生物区系Key words
Litopenaeus vannamei/Vibrio/phage cocktail/microbiota分类
农业科技引用本文复制引用
翁歆之,吕孙建,刁奕昕,贺洁,刘莉,沈海钰,郭琦,沈卫锋,韩明明,楼宝..弧菌噬菌体鸡尾酒制剂对凡纳滨对虾肠道微生物区系的影响[J].浙江农业学报,2025,37(5):1045-1056,12.基金项目
浙江省产业团队项目"设施化养殖凡纳滨对虾生物安保体系构建与示范" （）
浙江省重点研发计划(2022C02027) （2022C02027）
