中国肿瘤生物治疗杂志2025，Vol.32Issue(6)：620-627,8.DOI:10.3872/j.issn.1007-385x.2025.06.008
吲哚胺2,3-双加氧酶1在不同类型乳腺癌中的表达及其与患者预后和免疫细胞浸润的关系
Expression of indoleamin 2,3-dioxygenase 1 in different types of breast cancer and its relationship with patient prognosis and immune cell infiltration
摘要
Abstract
Objective:To investigate the expression of indoleamine 2,3-dioxygenase 1(IDO1)in different types of breast cancer tissues and its relationship with patient prognosis and immune cell infiltration.Methods:RNA sequencing data of breast cancer and corresponding clinical information from the TCGA database were collected.The differential expression of IDO1 mRNA in various breast cancer tissues(different subtypes,stages,menopause statuses,and age groups)and adjacent normal tissues were analyzed.Breast cancer patients with significant differences in IDO1 mRNA expression were divided into high and low expression groups,and their disease-specific survival(DSS)was compared between the two groups.The relationship between IDO1 mRNA expression and immune cell infiltration in cancer tissues with significant DSS differences was analyzed.Immunohistochemistry was used to detect IDO1 protein expression in ER-negative,PR-negative,HER2-positive,and stage Ⅱ breast cancer tissues,to verify the data from the database.Results:IDO1 mRNA was highly expressed in breast cancer tissues but varied across different breast cancer types.IDO1 mRNA was highly expressed in breast cancer tissues of patients with ER-negative,PR-negative,HER2-positive,HER2-negative subtypes,stage Ⅱ,T2 stage,N0 stage,M0 stage,premenopausal,postmenopausal,and age≤60 years(P<0.05 or P<0.01,or P<0.001).In the ER-negative,PR-negative,HER2-positive,and stage Ⅱ subgroups,breast cancer tissues patients with high IDO1 mRNA expression had significantly higher DSS than those with low expression(P<0.05 or P<0.01).In ER-negative,PR-negative,HER2-positive,and stageⅡ breast cancer tissues,IDO1 mRNA expression was associated with immune cell infiltration,including activated dendritic cells(aDCs),Th1 cells,T cells,CD56dim NK cells,CTLs,and Treg cells(all P<0.001).IDO1 protein was highly expressed in ER-negative,PR-negative,HER2-positive,and stage Ⅱ breast cancer tissues(all P<0.001),consistent with the data from the database.Conclusion:IDO1 expression varies across different types of breast cancer tissues.The expression of IDO1 is associated with the prognosis and immune cell infiltration in ER-negative,PR-negative,HER2-positive,and at stage Ⅱ breast cancer patients.关键词
乳腺癌/吲哚胺2,3-双加氧酶1(IDO1)/预后/免疫浸润Key words
breast cancer/indoleamine 2,3-dioxygenase 1(IDO1)/prognosis/immune infiltration分类
医药卫生引用本文复制引用
李小飘,李洁,肖俊,董宇华,何迅,张金娟..吲哚胺2,3-双加氧酶1在不同类型乳腺癌中的表达及其与患者预后和免疫细胞浸润的关系[J].中国肿瘤生物治疗杂志,2025,32(6):620-627,8.基金项目
贵州省卫生健康委科学技术基金(No.gzwkj2022-229) （No.gzwkj2022-229）
贵州省省级科技计划(黔科合基础[2020]1Y344) （黔科合基础[2020]1Y344）
