中国临床药理学杂志2025，Vol.41Issue(2)：193-197,5.DOI:10.13699/j.cnki.1001-6821.2025.02.009
miR-508-3p通过调控ZEB1参与卵巢癌进展的研究
Research of miR-508-3p involvement in ovarian cancer progression by regulating ZEB1
摘要
Abstract
Objective To investigate the expression of microRNA-508-3p(miR-508-3p)in epithelial ovarian cancer(EOC)tissue,its impact on the migration and invasion of ovarian cancer cells,and its regulatory relationship with zinc-finger E-box-binding homeobox 1(ZEB1).Methods The surgical resection of EOC cancer tissues and paired adjacent normal tissues were collected.SKOV3 cells were divided into the NC mimic group(transfected with NC mimic),miR-508-3p mimic group(transfected with miR-508-3p mimic),si-NC group(transfected with si-NC),si-ZEB1 group(transfected with si-ZEB1)and co-transfection group(co-transfected with si-ZEB1 and miR-508-3p mimic).The mRNA expression levels of miR-508-3p and ZEB1 in EOC cancer tissues,adjacent normal tissues and five groups of cells were measured by real-time quantitative polymerase chain reaction.The Transwell assay was used to detect the cell migration and invasion abilities.Results The relative expression levels of miR-508-3p in EOC tissues and adjacent normal tissues were 0.77±0.36 and 1.07±0.40,the relative expression levels of ZEB1 mRNA in EOC tissues and adjacent normal tissues were 2.10±1.21 and 1.29±0.95,and the differences were statistically significant(all P＜0.01).The migration cell number of the NC mimic,miR-508-3p mimic,si-NC,si-ZEB1 and co-transfection groups was 633.00±32.49,319.20±19.89,650.40±25.85,375.00±17.25 and 129.40±17.10;the invasion cell number was 527.20±25.01,288.60±16.68,520.00±25.83,293.40±18.37 and 76.60±8.76;the relative expression levels of miR-508-3p were 1.05±0.37,3.94±1.21,1.01±0.21,1.26±0.34 and 3.40±0.41;the relative expression levels of ZEB1 mRNA were 1.00±0.04,0.58±0.05,1.00±0.08,0.54±0.07 and 0.29±0.03,respectively.The above indicators showed statistically significant differences between the miR-508-3p mimic group and the NC mimic group,between the si-NC group and the co-transfection group(P＜0.01,P＜0.05).Conclusion MiR-508-3p is lowly expressed in EOC cancer tissue,and it may inhibit the migration and invasion of ovarian cancer cells by targeting ZEB1 expression.关键词
微小RNA-508-3p/锌指E盒结合蛋白1/上皮性卵巢癌/迁移/侵袭Key words
microRNA-508-3p/zinc-finger E-box-binding homeobox 1/epithelial ovarian cancer/migration/invasion分类
医药卫生引用本文复制引用
徐玉红,朱帅莹,单江静,郑伟平,张慧雅,王运根..miR-508-3p通过调控ZEB1参与卵巢癌进展的研究[J].中国临床药理学杂志,2025,41(2):193-197,5.基金项目
浙江省医药卫生科技计划基金资助项目(2023KY1250、2023KY362) （2023KY1250、2023KY362）
