中国临床药理学杂志2025，Vol.41Issue(2)：198-202,5.DOI:10.13699/j.cnki.1001-6821.2025.02.010
基于NF-κB/NLRP3/caspase-1信号轴探讨牛膝皂苷I抑制软骨细胞焦亡的研究
Research of Achyranthoside I inhibiting pyroptosis in chondrocytes based on the NF-κB/NLRP3/caspase-1 signaling axis
摘要
Abstract
Objective To investigate the mechanism of Achyranthoside I inhibits pyroptosis in chondrocytes through the nuclear factor-κB(NF-κB)/NOD receptor protein structure domain related proteins 3(NLRP3)/cystine containing aspartate specific proteins-1(caspase-1)signaling pathway.Methods Primary mouse chondrocytes were divided into blank group(phosphate buffered solution with the same volume),model group[10 ng•mL-1 interleukin-1β(IL-1 β)],control group(10 ng•mL-1 IL-1 β+20 μmol•L-1 celecoxib)and experimental group(10 ng•mL-1 IL-1 β+3 μg•mL-1 Achyranthoside I).After 24 hours of intervention,the cell proliferation was measured by cell counting kit 8,the levels of superoxide dismutase(SOD),malondialdehyde(MDA),IL-1 and IL-6 were detected by enzyme-linked immunosorbent assay,the protein expression levels of NF-κB p65,NLRP3 and caspase-1 were detected by Western Blot.Results The apoptosis rates in experimental,control,model and blank groups were(13.34±0.61)％,(15.64±1.01)％,(21.81±1.10)％and 0;the SOD levels were(147.03±16.49),(130.09±7.33),(122.03±10.71)and(164.40±22.74)nU•mL-1;the MDA levels were(6.43±0.71),(7.63±1.01),(8.89±1.84)and(5.69±0.81)nmol•L-1;the IL-1 levels were(338.69±40.95),(361.78±32.15),(391.44±30.59)and(289.23±25.19)pg•mL-1;the IL-6 levels were(89.96±8.81),(101.10±11.59),(120.39±14.71)and(60.29±6.03)pg•mL-1;the relative expression levels of NF-κB p65 were 0.68±0.05,0.97±0.05,1.26±0.05 and 0.57±0.05;the relative expression levels of NLRP3 were 0.71±0.08,1.02±0.10,1.50±0.06 and 0.31±0.05;the relative expression levels of caspase-1 were 0.70±0.07,1.29±0.08,1.66±0.07 and 0.51±0.07,respectively.Compared with the model group,the differences of above indexes were statistically significant in the experimental group(all P＜0.05).Conclusion Achyranthoside I can improve the oxidative stress status induced by IL-1 β in chondrocytes,reduce the expression of proteins related to the NF-κB signaling pathway,and thereby decrease the occurrence of caspase-1 dependent pyroptosis,providing a protective effect on chondrocytes.关键词
牛膝皂苷I/软骨细胞/氧化应激/半胱氨酸蛋白酶-1依赖性/细胞焦亡Key words
Achyranthoside I/chondrocytes/oxidative stress/caspase-1-dependent/pyroptosis分类
医药卫生引用本文复制引用
刘泽萱,韩易言,关雪峰,张宇,戴俭宇..基于NF-κB/NLRP3/caspase-1信号轴探讨牛膝皂苷I抑制软骨细胞焦亡的研究[J].中国临床药理学杂志,2025,41(2):198-202,5.基金项目
国家重点研发计划基金资助项目(2021YFC1712800) （2021YFC1712800）
国家资助博士后研究人员计划基金资助项目(GZC20231025) （GZC20231025）
辽宁省科学技术计划项目创新能力提升联合基金资助项目(2022-NLTS-13-02) （2022-NLTS-13-02）
辽宁省教育厅基本科研基金资助项目(JYTQN2023458) （JYTQN2023458）
