临床与病理杂志2025，Vol.45Issue(3)：265-274,10.DOI:10.11817/j.issn.2095-6959.2025.240697
甲状腺乳头状癌中GJB4和ADRA1B的异常表达:影响预后的关键因子
Abnormal expression of GJB4 and ADRA1B in papillary thyroid carcinoma:Critical factors influencing prognosis
摘要
Abstract
Objective:Papillary thyroid carcinoma(PTC)is a common endocrine malignancy,and its prognosis is often poor in advanced stages.This study aims to investigate the expression of gap junction protein beta 4(GJB4)and adrenergic receptor alpha 1B(ADRA1B)in PTC tissues and to analyze their correlation with patient prognosis,in order to explore their roles in PTC progression and potential as prognostic biomarkers. Methods:Tumor and adjacent non-tumor tissues from 80 PTC patients were collected.Immunohistochemistry was used to semi-quantitatively assess the expression levels of GJB4 and ADRA1B.Western blotting was used to evaluate protein levels,and real-time reverse transcription PCR(real-time RT-PCR)was performed to determine mRNA expression.Clinical data,including gender,age,tumor size,and stage,were collected from hospital records.Patients were followed for 2 years postoperatively,with recurrence and central lymph node metastasis recorded.Kaplan-Meier survival analysis was conducted to evaluate the prognostic value of GJB4 and ADRA1B expression. Results:Immunohistochemistry revealed that GJB4 and ADRA1B protein levels were significantly higher in tumor tissues compared to adjacent tissues(P<0.05).Western blotting and real-time RT-PCR confirmed significantly elevated protein and mRNA levels of GJB4 and ADRA1B in tumor tissues(all P<0.05).Among the 80 PTC patients,26(32.5%)experienced recurrence or central lymph node metastasis.GJB4 and ADRA1B expression levels were significantly higher in patients with lymph node metastasis than those without(both P<0.05).Univariate analysis showed that expression of GJB4 and ADRA1B was not associated with gender or age(all P>0.05)but was significantly correlated with larger tumor size and higher pathological stage(all P<0.05).Kaplan-Meier analysis revealed significantly higher recurrence rates in patients with high GJB4 or ADRA1B expression(both P<0.05). Conclusion:GJB4 and ADRA1B are significantly overexpressed in PTC tissues,and their high expression is closely associated with poor prognosis.These findings suggest that GJB4 and ADRA1B may serve as key biomarkers and potential therapeutic targets for improving prognosis and preventing postoperative recurrence in PTC.关键词
甲状腺乳头状癌/间隙连接蛋白β4/肾上腺素能受体α1B/预后/关键因子Key words
papillary thyroid carcinoma/gap junction protein beta 4/adrenergic receptor alpha 1B/prognosis/critical factors引用本文复制引用
冀雅铭,殷玲,翟艳,陈红,王旭,孙吉瑞..甲状腺乳头状癌中GJB4和ADRA1B的异常表达:影响预后的关键因子[J].临床与病理杂志,2025,45(3):265-274,10.基金项目
保定市科技计划项目(2241ZF046).This work was supported by the Project of Baoding Science and Technology Planning,China(2241ZF046). （2241ZF046）
